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anti-NOS3; cNOS; NOS III; Nitric oxide synthase (endothelial) antibody :: Rabbit anti-Mouse, Rat eNOS Polyclonal Antibody

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Catalog # MBS565304
Unit / Price
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  0.05 mg  /  $290 +1 FREE 8GB USB
Western Blot (WB)
Product Name

eNOS (NOS3; cNOS; NOS III; Nitric oxide synthase (endothelial)), Polyclonal Antibody

Full Product Name

eNOS polyclonal antibody

Product Synonym Names
eNOS
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
163729
3D Structure
ModBase 3D Structure for P29474
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
Specificity
Recognizes mouse and rat eNOS. Detects a band of ~135kDa by Western blot.
Purity/Purification
Peptide Affinity Purified
Form/Format
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Immunogen
Synthetic peptide corresponding to a portion of human eNOS.
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-NOS3; cNOS; NOS III; Nitric oxide synthase (endothelial) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.
Immunohistochemistry (IHC) (PS), Western Blot (WB)
Western Blot (1:500, ECL)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~135kDa by Western blot.

Western blot analysis: Lane 1: Mouse brain, Lane 2: Rat brain; probed with eNOS pAb .
anti-NOS3; cNOS; NOS III; Nitric oxide synthase (endothelial) antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NOS3; cNOS; NOS III; Nitric oxide synthase (endothelial). It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
67,876 Da
NCBI Official Full Name
Nitric oxide synthase, endothelial
NCBI Official Synonym Full Names
nitric oxide synthase 3
NCBI Official Symbol
NCBI Official Synonym Symbols
eNOS; ECNOS
  [Similar Products]
NCBI Protein Information
nitric oxide synthase, endothelial
UniProt Protein Name
Nitric oxide synthase, endothelial
UniProt Synonym Protein Names
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
UniProt Gene Name
UniProt Synonym Gene Names
cNOS; eNOS; NOSIII  [Similar Products]
UniProt Entry Name
NOS3_HUMAN
NCBI Summary for NOS3; cNOS; NOS III; Nitric oxide synthase (endothelial)
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
UniProt Comments for NOS3; cNOS; NOS III; Nitric oxide synthase (endothelial)
eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; Golgi membrane; nucleus; plasma membrane

Molecular Function: actin monomer binding; cadmium ion binding; calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding

Biological Process: angiogenesis; arginine catabolic process; blood coagulation; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; mitochondrion organization and biogenesis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of potassium ion transport; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; nitric oxide metabolic process; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood pressure; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to heat; smooth muscle hyperplasia; vascular endothelial growth factor receptor signaling pathway

Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Diseases associated with anti-NOS3; cNOS; NOS III; Nitric oxide synthase (endothelial) antibody
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