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anti-IRAK3 antibody :: Goat IRAK3 Polyclonal Antibody

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Catalog # MBS421426
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

IRAK3, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-IRAK3 (mouse) Antibody

Product Synonym Names
IRAK3; interleukin-1 receptor-associated kinase 3; ASRT5; IRAK-M; IRAKM; interleukin-1 receptor-associated kinase M; IRAK3 antibody; interleukin-1 receptor-associated kinase 3 antibody; ASRT5 antibody; IRAK-M antibody; IRAKM antibody; interleukin-1 receptor-associated kinase M antibody; IRAK3 (mouse)
Antibody/Peptide Pairs
IRAK3 peptide (MBS425489) is used for blocking the activity of IRAK3 antibody (MBS421426)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
GHSYGSKPME KR
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen
Peptide with sequence C-GHSYGSKPMEKR, from the internal region (near the C Terminus) of the protein sequence according to NP_082955.2.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-IRAK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-IRAK3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-IRAK3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.

Western Blot: Approx 65kDa band observed in Rat Liver lysates (calculated MW of 67.8kDa according to NP_082955.2).
An approx 70kDa is observed in Mouse Bone Marrow lysates but this band disappears in lysates from the knock-out mouse.
Recommended concentration: 0. 05-0.2ug/ml.

Western Blot (WB) of anti-IRAK3 antibody
(0. 05 ug/ml) staining of Rat Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected with chemiluminescence.
anti-IRAK3 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-IRAK3 antibody
(0.5 ug/ml) staining of Mouse Bone Marrow (wildtype first lane, KO second lane) lysates (35 ug protein in RIPA buffer). Primary incubation was overnight at 4C Detected with chemiluminescence. Data obtained from anonymous customer
anti-IRAK3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for IRAK3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
interleukin-1 receptor-associated kinase 3
NCBI Official Synonym Full Names
interleukin-1 receptor-associated kinase 3
NCBI Official Symbol
Irak3  [Similar Products]
NCBI Official Synonym Symbols
IRAK-M; AI563835; 4833428C18Rik
  [Similar Products]
NCBI Protein Information
interleukin-1 receptor-associated kinase 3
UniProt Protein Name
Interleukin-1 receptor-associated kinase 3
UniProt Synonym Protein Names
IL-1 receptor-associated kinase M; IRAK-M
UniProt Gene Name
Irak3  [Similar Products]
UniProt Synonym Gene Names
IRAK-3; IRAK-M  [Similar Products]
UniProt Entry Name
IRAK3_MOUSE
UniProt Comments for IRAK3
IRAK3: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; IRAK family

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; kinase activity; magnesium ion binding; nucleotide binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity; transferase activity

Biological Process: activation of NF-kappaB transcription factor; apoptosis; cytokine and chemokine mediated signaling pathway; inhibition of NF-kappaB transcription factor; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of innate immune response; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of MAP kinase activity; negative regulation of protein catabolic process; negative regulation of protein complex disassembly; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of protein complex disassembly; response to exogenous dsRNA; response to lipopolysaccharide; response to peptidoglycan; response to virus; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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