• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-GRIN1 antibody :: Rabbit anti-Mouse, rat NMDA NR1 Subunit, Splice Variant C1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS502079
Unit / Price
  0.025 mg  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

NMDA NR1 Subunit, Splice Variant C1 (GRIN1), Polyclonal Antibody

Popular Item
Also Known As

Anti-NMDA Receptor, NR1 Subunit, C1 Splice Variant Insert

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
959
Chromosome Location
Chromosome: 3; NC_005102.3 (2489158..2516082, complement). Location: 3p13
3D Structure
ModBase 3D Structure for P35439
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, rat
Specificity
Specific for the ~120k NR1 subunit of the NMDA receptor containing the C1 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C1 insert.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)
Form/Format
25 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody sh
Antigen
Peptide from the NR1 subunit, C1 splice variant insert of the NMDA receptor.
Immunogen Information
Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C1 splice variant insert conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Bovine, canine, human, non-human primates
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. It is anticipated that the antibody will also work with bovine, canine, human, and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory and neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-GRIN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GRIN1 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-GRIN1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GRIN1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (frozen sections; unpublished observations): 1:1000 to 1:2000

Testing Data of anti-GRIN1 antibody
anti-GRIN1 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for GRIN1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
120
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 1 isoform 1b
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 1
NCBI Official Symbol
Grin1  [Similar Products]
NCBI Official Synonym Symbols
NR1; GluN1; NMDAR1
  [Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, NMDA 1; NMD-R1; neurotransmitter receptor; NMDA R1 receptor C1 cassette; N-methyl-D-aspartate glutamate receptor; glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 1
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1
UniProt Gene Name
Grin1  [Similar Products]
UniProt Synonym Gene Names
Nmdar1; GluN1; NMD-R1  [Similar Products]
UniProt Entry Name
NMDZ1_RAT
NCBI Summary for GRIN1
subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006]
UniProt Comments for GRIN1
Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. Ref.18

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked

By similarity. Found in a complex with GRIN2A or GRIN2B, GRIN3A and PPP2CB. Found in a complex with GRIN2A or GRIN2B and GRIN3B. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes

By similarity. Interacts with DLG4 and MPDZ. Interacts with LRFN1 and LRFN2. Interacts with MYZAP. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.17

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Cell junction › synapse › postsynaptic cell membrane

By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density

By similarity. Note: Enriched in postsynaptic plasma membrane and postsynaptic densities

By similarity.

Post-translational modification: NMDA is probably regulated by C-terminal phosphorylation of an isoform ofNR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. Ref.7 Ref.12

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. [View classification]
Product References and Citations for anti-GRIN1 antibody
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Foldes RL, Rampersad V, Kamboj RK (1994) Cloning and sequence analysis of additional splice variants encoding human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303-304.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358
• Zukin RS, Bennett MVL (1995) Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci 18:306-313.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-GRIN1 antibody
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000