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anti-ORAI1 antibody :: Rabbit anti-Mouse, Rat ORAI1 Polyclonal Antibody

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Catalog # MBS222945
Unit / Price
  0.1 mg  /  $400 +1 FREE 8GB USB
Product Name

ORAI1, Polyclonal Antibody

Also Known As

RABBIT ANTI ORAI1 (C-TERMINAL)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
301
OMIM
610277
3D Structure
ModBase 3D Structure for Q96D31
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Specificity
This item recognises human ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kDa multi-pass membrane protein belonging to the ORAI family.

Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.

ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor.

Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry.

This item is not expected to cross react with either ORAI2 or ORAI3.
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (lot specific)
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 16 amino acid peptide from near the carboxy terminus of human ORAI1.
Buffer Solution
Antiserum Preparation
Antiserum to human ORAI1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-ORAI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ORAI1 antibody
MBS222945 recognises human ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kDa multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry. MBS222945 is not expected to cross react with either ORAI2 or ORAI3.
Applications Tested/Suitable for anti-ORAI1 antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-ORAI1 antibody
Western Blot: This item detects a band of approximately 49kDa in 49 cell lysates.
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1ug/ml

Testing Data #1 of anti-ORAI1 antibody
Western blot analysis of human ovary probed with Rabbit anti Human ORAI1 (MBS222945) at 0.5(A), 1(B) and 2(C) ug/ml
anti-ORAI1 antibody Testing Data #1 image
Testing Data #2 of anti-ORAI1 antibody
Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human ORAI1 (MBS222945) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
anti-ORAI1 antibody Testing Data #2 image
NCBI/Uniprot data below describe general gene information for ORAI1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,337 Da
NCBI Official Full Name
calcium release-activated calcium channel protein 1
NCBI Official Synonym Full Names
ORAI calcium release-activated calcium modulator 1
NCBI Official Symbol
ORAI1  [Similar Products]
NCBI Official Synonym Symbols
IMD9; TAM2; ORAT1; CRACM1; TMEM142A
  [Similar Products]
NCBI Protein Information
calcium release-activated calcium channel protein 1; calcium release-activated calcium modulator 1; protein orai-1; transmembrane protein 142A
UniProt Protein Name
Calcium release-activated calcium channel protein 1
UniProt Synonym Protein Names
Protein orai-1; Transmembrane protein 142A
UniProt Gene Name
ORAI1  [Similar Products]
UniProt Synonym Gene Names
CRACM1; TMEM142A  [Similar Products]
UniProt Entry Name
CRCM1_HUMAN
NCBI Summary for ORAI1
The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1). [provided by RefSeq, Sep 2011]
UniProt Comments for ORAI1
ORAI1: Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. Interacts with STIM1 and STIM2. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and STIM1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Belongs to the Orai family.

Protein type: Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: protein complex; membrane; integral to plasma membrane

Molecular Function: calmodulin binding; identical protein binding; protein binding; store-operated calcium channel activity

Biological Process: immune system process; regulation of calcium ion transport; positive regulation of calcium ion transport; blood coagulation

Disease: Myopathy, Tubular Aggregate, 2; Immunodeficiency 9
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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