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anti-S100A9 antibody :: anti-Mouse, Rat S100A9 Polyclonal Antibody

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Catalog # MBS177733
Unit / Price
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  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

S100A9, Polyclonal Antibody

Popular Item
Full Product Name

Anti-S100A9 Antibody

Product Synonym Names
Protein S100-A9; Leukocyte L1 complex heavy chain; 60B8AG; AW546964; BEE22; CAGB; Calgranulin B; Calgranulin-B; Calprotectin L1H subunit; CFAG; CGLB; Cystic fibrosis antigen; Cystic fibrosis antigen B; HGNC:10499; L1AG; Leukocyte L1 complex heavy chain; LIAG; MAC 387; MAC387; MIF; Migration inhibitory factor related protein 14; Migration inhibitory factor-related protein 14; MRP 14; MRP 8; MRP-14; MRP14; Myeloid-related protein 14; NIF; OTTHUMP00000015331; P14; Protein S100-A9; S100 A9; S100 calcium binding protein A9; S100 calcium binding protein A9 calgranulin B; S100 calcium-binding protein A9; S100A9; S10A9_HUMAN; S100 calcium binding protein A9
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P31725
Clonality
Polyclonal
Isotype
Rabbit IgG
Species Reactivity
Mouse, Rat
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Immunogen
E Coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8% and 78.6% amino acid (aa) sequence identity with human and rat S100A9, respectively.
Reconstitution
0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time.
Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-S100A9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-S100A9 antibody
Description: Rabbit IgG polyclonal antibody for Protein S100-A9(S100A9) detection. Tested with WB, IHC-P, ELISA in Mouse;Rat.

Background: S100 calcium-binding protein A9 (S100A9), also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B, is a protein that in humans is encoded by the S100A9 gene. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. And S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase.
Applications Tested/Suitable for anti-S100A9 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes for anti-S100A9 antibody
ELISA:
Concentration: 0.1-0.5ug/ml
Tested Species: Ms
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Ms
Immunohistochemistry (IHC) Paraffin-embedded sections
Concentration: 0.5-1ug/ml
Tested Species: Ms, Rat
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.

Other applications have not been tested.
Optimal dilutions should be determined by end users.

Western Blot (WB) of anti-S100A9 antibody
Anti- S100A9 Picoband antibody, MBS177733, Western blotting
All lanes: Anti S100A9 (MBS177733) at 0.5ug/ml
WB: Mouse Ovary Tissue Lysate at 50ug
Predicted bind size: 13KD
Observed bind size: 13KD
anti-S100A9 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-S100A9 antibody
Anti- S100A9 Picoband antibody, MBS177733, IHC(P)
IHC(P): Mouse Spleen Tissue
anti-S100A9 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-S100A9 antibody
Anti- S100A9 Picoband antibody, MBS177733, IHC(P)
IHC(P): Rat Spleen Tissue
anti-S100A9 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for S100A9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
13,049 Da
NCBI Official Full Name
protein S100-A9
NCBI Official Synonym Full Names
S100 calcium binding protein A9 (calgranulin B)
NCBI Official Symbol
S100a9  [Similar Products]
NCBI Official Synonym Symbols
p14; Cagb; GAGB; L1Ag; BEE22; MRP14; 60B8Ag; AW546964
  [Similar Products]
NCBI Protein Information
protein S100-A9
UniProt Protein Name
Protein S100-A9
UniProt Synonym Protein Names
Calgranulin-B; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9
Protein Family
UniProt Gene Name
S100a9  [Similar Products]
UniProt Synonym Gene Names
Cagb; Mrp14; MRP-14; p14  [Similar Products]
UniProt Entry Name
S10A9_MOUSE
UniProt Comments for S100A9
S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Cellular Component: cytoplasm; cytoskeleton; extracellular region; extracellular space; membrane; nucleus; plasma membrane

Molecular Function: antioxidant activity; arachidonic acid binding; calcium ion binding; metal ion binding; microtubule binding; RAGE receptor binding; zinc ion binding

Biological Process: actin cytoskeleton reorganization; apoptosis; astrocyte development; autophagy; caspase activation; chemotaxis; immune system process; inflammatory response; innate immune response; leukocyte chemotaxis; leukocyte migration during inflammatory response; neutrophil chemotaxis; peptidyl-cysteine S-nitrosylation; positive regulation of blood coagulation; positive regulation of inflammatory response; positive regulation of peptide secretion; regulation of inflammatory response; regulation of integrin biosynthetic process; regulation of translation
Product References and Citations for anti-S100A9 antibody
1. "Entrez Gene: S100A9 S100 calcium binding protein A9". 2. Cheng P, Corzo CA, Luetteke N, Yu B, Nagaraj S, Bui MM, Ortiz M, Nacken W, Sorg C, Vogl T, Roth J, Gabrilovich DI (September 2008). "Inhibition of dendritic cell differentiation and accumulation of myeloid-derived suppressor cells in cancer is regulated by S100A9 protein". J. Exp. Med. 205 (10): 2235-49.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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