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anti-SFTPA1 antibody :: Rabbit anti-Mouse, Rat Surfactant Protein A Polyclonal Antibody

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Catalog # MBS175612
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

Surfactant Protein A (SFTPA1), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Anti-SFTPA1 Antibody

Product Synonym Names
Pulmonary surfactant-associated protein A; surfactant protein A1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
248
3D Structure
ModBase 3D Structure for P35242
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Mouse, Rat
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Surfactant Protein A (233-248aa WNDKGCLQYRLAICEF), identical to the related rat sequence.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-SFTPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SFTPA1 antibody
Description: Rabbit IgG polyclonal antibody for Pulmonary surfactant-associated protein A(SFTPA1) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: Surfactant protein A is an innate immune system collectin. The gene encoding SP-A map to mouse chromosome 14. Surfactant protein-A, which plays a role in innate host defense in the lung, is also expressed in the Eustachian tube. We report that the frequency of specific surfactant protein-A haplotypes and genotypes differs between children with recurrent otitis media compared with a control population.
Applications Tested/Suitable for anti-SFTPA1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-SFTPA1 antibody
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Ms, Rat

Immunohistochemistry (Paraffin-emmbedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Rat
Predicted Species: Ms
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determiend by end users.

Western Blot (WB) of anti-SFTPA1 antibody
Figure 1. Western blot analysis of SFTPA1 using anti- SFTPA1 antibody (MBS175612).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SFTPA1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26KD. The expected band size for SFTPA1 is at 26KD.
anti-SFTPA1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SFTPA1 antibody
Figure 2. IHC analysis of SFTPA1 using anti- SFTPA1 antibody (MBS175612).
SFTPA1 was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- SFTPA1 Antibody (MBS175612) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-SFTPA1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SFTPA1 antibody
Figure 3. IHC analysis of SFTPA1 using anti- SFTPA1 antibody (MBS175612).
SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- SFTPA1 Antibody (MBS175612) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-SFTPA1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for SFTPA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Pulmonary surfactant-associated protein A
NCBI Official Synonym Full Names
surfactant associated protein A1
NCBI Official Symbol
Sftpa1  [Similar Products]
NCBI Official Synonym Symbols
SP-A; Sftp1; Sftp-1
  [Similar Products]
NCBI Protein Information
pulmonary surfactant-associated protein A; PSAP; PSP-A; surfactant pulmonary associated protein A1
UniProt Protein Name
Pulmonary surfactant-associated protein A
UniProt Gene Name
Sftpa1  [Similar Products]
UniProt Synonym Gene Names
Sftp-1; Sftp1; Sftpa; PSAP; PSP-A; SP-A  [Similar Products]
UniProt Entry Name
SFTPA_MOUSE
UniProt Comments for SFTPA1
SFTPA2: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Defects in SFTPA2 are a cause of pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. Belongs to the SFTPA family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: multivesicular body; extracellular space; rough endoplasmic reticulum; cytoplasmic vesicle

Biological Process: positive regulation of phagocytosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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