View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Goat anti Mouse IgM (Fc specific) Secondary Antibody Catalog # MBS571337 Unit / Price 1 mL / $760 +1 FREE 8GB USB 5x1 mL / $3,250 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Goat anti Mouse IgM (Fc specific), Secondary Antibody Popular Item Full Product Name Goat anti Mouse IgM (Fc specific), conjugated with Horseradish peroxidase Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Goat Species Reactivity Mouse Specificity Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgM, Fc specific Source Purified homogenous IgM isolated from pooled mouse serum. Immunization with intact ( 19S) abd split IgM (7S). Freund's complete adjuvant is used in the first step of the immunization procedure. Procedure Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 degree C. Cross Reactivity Note Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial diffusion a reaction with Rat IgM has been observed. Preparation and Storage The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 degree C; prolonged storage at or below -20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Other Notes Small volumes of Goat anti Mouse IgM (Fc specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Goat anti Mouse IgM (Fc specific) secondary antibody The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with purified IgG, IgG/Fab fragments and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Product Categories/Family for Goat anti Mouse IgM (Fc specific) secondary antibody Conjugated Secondary Antibody Applications Tested/Suitable for Goat anti Mouse IgM (Fc specific) secondary antibody ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC)-Paraffin, Dot Blot (DB), Immunoblotting (IB) TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP