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anti-ANGPT1 antibody :: Rabbit anti-Murine Angiopoietin 1 Polyclonal Antibody

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Catalog # MBS534326
Unit / Price
  0.1 mg  /  $710 +1 FREE 8GB USB
anti-ANGPT1 antibody
Product Name

Angiopoietin 1 (ANGPT1), Polyclonal Antibody

Also Known As

Angiopoietin 1 antibody

Product Synonym Names
Polyclonal Angiopoietin 1; Anti-Angiopoietin 1; AGP 1; AGP1; Angiopoietin -1; AGPT; Angiopoietin 1; ANG 1; KIAA0003; ANGPT1; ANG1; Angiopoietin1; ANGPT 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
498
OMIM
601667
3D Structure
ModBase 3D Structure for Q15389
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Murine
Purity/Purification
Angiopoietin 1 antibody was purified by affinity chromatography.
Form/Format
Lyophilized from PBS with 0.1% BSA. Reconstitute in PBS to a concentration of 1 mg/ml
Biological Significance
Angiopoietin-1 is an angiogenic secreted protein that interacts with endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells.
Immunogen
Angiopoietin 2 antibody was raised in rabbit using 20-aa peptide from mouse Ang-1 as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-ANGPT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ANGPT1 antibody
Affinity purified Rabbit polyclonal Angiopoietin 1 antibody
Applications Tested/Suitable for anti-ANGPT1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ANGPT1 antibody
ELISA: 1:10,000-1:100,000 (50-100 ng of control peptide/well)
IHC: 2-20 ug/ml
IHC: 2-20 ug/ml
WB: 1-10 ug/ml
NCBI/Uniprot data below describe general gene information for ANGPT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,456 Da[Similar Products]
NCBI Official Full Name
angiopoietin-1 isoform 1
NCBI Official Synonym Full Names
angiopoietin 1
NCBI Official Symbol
ANGPT1  [Similar Products]
NCBI Official Synonym Symbols
AGP1; AGPT; ANG1
  [Similar Products]
NCBI Protein Information
angiopoietin-1
UniProt Protein Name
Angiopoietin-1
Protein Family
UniProt Gene Name
ANGPT1  [Similar Products]
UniProt Synonym Gene Names
KIAA0003; ANG-1  [Similar Products]
UniProt Entry Name
ANGP1_HUMAN
NCBI Summary for ANGPT1
Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]
UniProt Comments for ANGPT1
ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q23.1

Cellular Component: extracellular space; microvillus; plasma membrane; extracellular region; lipid raft

Molecular Function: receptor tyrosine kinase binding

Biological Process: transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of cell adhesion; positive regulation of receptor internalization; negative regulation of protein import into nucleus; negative regulation of cell adhesion; negative regulation of protein amino acid phosphorylation; positive chemotaxis; hemopoiesis; negative regulation of neuron apoptosis; cell differentiation; regulation of I-kappaB kinase/NF-kappaB cascade; Tie receptor signaling pathway; regulation of endothelial cell proliferation; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; negative regulation of cytokine secretion during immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; heparin biosynthetic process; positive regulation of protein ubiquitination; regulation of protein binding; negative regulation of vascular permeability; regulation of tumor necrosis factor production; sprouting angiogenesis; regulation of satellite cell proliferation; blood coagulation; leukocyte migration; negative regulation of apoptosis
Precautions
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Disclaimer
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