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anti-EGF antibody :: Rabbit Epidermal Growth Factor (EGF) Polyclonal Antibody

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Catalog # MBS2006805 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $120 +1 FREE 8GB USB
  0.02 mg  /  $140 +1 FREE 8GB USB
  0.05 mg  /  $200 +1 FREE 8GB USB
  0.1 mg  /  $260 +1 FREE 8GB USB
  0.2 mg  /  $400 +1 FREE 8GB USB
  1 mg  /  $950 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Epidermal Growth Factor (EGF), Polyclonal Antibody

Popular Item
Also Known As

Biotin-Linked Antibody to Epidermal Growth Factor (EGF)

Product Gene Name
Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
53
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH
Chromosome Location
Chromosome: 4; NC_000004.11 (110834040..110934118). Location: 4q25
OMIM
131530
3D Structure
ModBase 3D Structure for P01133
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Porcine (Pig)
Specificity
The antibody is a rabbit polyclonal antibody raised against EGF conjugated to biotin. It has been selected for its ability to recognize EGF in immunohistochemical staining andwestern blotting.
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol
Concentration
200ug/ml (lot specific)
Source
Antibody labeling
Traits
Liquid
Unconjugated Antibody
The unconjugated antibody version of this item is also available as catalog #MBS2002325
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of anti-EGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-EGF antibody
Applications Tested/Suitable for anti-EGF antibody
WB; ICC; IHC-P; IHC-F; IF; ELISA.
Application Notes for anti-EGF antibody
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-EGF antibody
(Figure. Western Blot; Sample: Recombinant protein.)
anti-EGF antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
133,994 Da
NCBI Official Full Name
epidermal growth factor
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
NCBI Official Synonym Symbols
URG; HOMG4
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NCBI Protein Information
pro-epidermal growth factor; beta-urogastrone
UniProt Protein Name
Pro-epidermal growth factor
UniProt Synonym Protein Names
Urogastrone
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
EGF_HUMAN
NCBI Summary for EGF
This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Comments for EGF
EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Membrane protein, integral; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space; lysosomal membrane; integral to membrane; plasma membrane; extracellular region

Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; transmembrane receptor protein tyrosine kinase activation (dimerization); peptidyl-tyrosine phosphorylation; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; signal transduction; positive regulation of MAP kinase activity; platelet degranulation; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; innate immune response; negative regulation of secretion; angiogenesis; blood coagulation; DNA replication; positive regulation of phosphorylation; positive regulation of DNA binding; STAT protein nuclear translocation; positive regulation of granule cell precursor proliferation

Disease: Hypomagnesemia 4, Renal
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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