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anti-HSD11b1 antibody :: Rabbit anti-Rat 11-Beta-Hydroxysteroid Dehydrogenase Polyclonal Antibody

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Catalog # MBS2026098 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $115 +1 FREE 8GB USB
  0.02 mg  /  $135 +1 FREE 8GB USB
  0.05 mg  /  $190 +1 FREE 8GB USB
  0.1 mg  /  $245 +1 FREE 8GB USB
  0.2 mg  /  $365 +1 FREE 8GB USB
  1 mg  /  $870 +1 FREE 8GB USB
Product Name

11-Beta-Hydroxysteroid Dehydrogenase (HSD11b1), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P16232
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Specificity
The antibody is a rabbit polyclonal antibody raised against HSD11b1. It has beenselected for its ability to recognize HSD11b1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
HSD11b1 (Met1~Asn288)
Organism Species
Rattus norvegicus (Rat)
Conjugate
No Conjugate
Immunogen
Recombinant HSD11b1 (Met1~Asn288) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2064425
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-HSD11b1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HSD11b1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-HSD11b1 antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-HSD11b1 antibody
Western Blot: Sample: Recombinant protein.
anti-HSD11b1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-HSD11b1 antibody
DABstainingonIHC-P.Samples:RatTissue)
anti-HSD11b1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for HSD11b1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
28,732 Da
NCBI Official Full Name
corticosteroid 11-beta-dehydrogenase isozyme 1
NCBI Official Synonym Full Names
hydroxysteroid 11-beta dehydrogenase 1
NCBI Official Symbol
Hsd11b1  [Similar Products]
NCBI Official Synonym Symbols
LRRGT00065
  [Similar Products]
NCBI Protein Information
corticosteroid 11-beta-dehydrogenase isozyme 1
UniProt Protein Name
Corticosteroid 11-beta-dehydrogenase isozyme 1
UniProt Synonym Protein Names
11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11-beta-HSD1
UniProt Gene Name
Hsd11b1  [Similar Products]
UniProt Synonym Gene Names
Hsd11; 11-DH; 11-beta-HSD1  [Similar Products]
NCBI Summary for HSD11b1
catalyzes the interconversion of cortisol and cortisone; plays a role in glucocorticoid metabolism; may regulate blood pressure [RGD, Feb 2006]
UniProt Comments for HSD11b1
HSD11B1: Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7- ketocholesterol to 7-beta-hydroxycholesterol. Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD). In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: EC 1.1.1.146; Endoplasmic reticulum; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; Membrane protein, integral; Nuclear envelope; Oxidoreductase

Chromosomal Location of Human Ortholog: 13q27

Cellular Component: apical part of cell; cytoplasm; endoplasmic reticulum membrane; integral component of membrane; intracellular membrane-bound organelle; membrane; nuclear membrane; rough endoplasmic reticulum

Molecular Function: 11-beta-hydroxysteroid dehydrogenase [NAD(P)] activity; NADP binding; steroid binding

Biological Process: glucocorticoid biosynthetic process; glucocorticoid catabolic process; lung development; regulation of pentose-phosphate shunt; response to nutrient levels
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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