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anti-AQP2 antibody :: Rabbit anti-Rat Aquaporin 2 (Ser 269) Polyclonal Antibody

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Catalog # MBS502165
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

Aquaporin 2 (Ser 269) (AQP2), Polyclonal Antibody

Popular Item
Full Product Name

Anti-Phospho-Ser269 Aquaporin 2

Product Synonym Names
Voltage-dependent T-type calcium channel subunit alpha-1I,CACNA1I,oltage-gated calcium channel subunit alpha Cav3.3,Cav3.3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: X; NC_005120.3 (115742312..115747347). Location: chromosome: X
3D Structure
ModBase 3D Structure for P34080
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Specificity
Specific for the ~29k AQP2 protein phosphorylated at Ser269. Also recognizes the glycosylated form of AQP2 at ~ 37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser269 of rat aquaporin 2.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269 conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Bovine, canine, mouse
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine and mouse tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al., 2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Serine-269 has been recently identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008).
Preparation and Storage
For long term storage -80 degree C is recommended, but shorter term storage at -20 degree C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solution are stable for a minimum of 1 year at -20 degree C.

Shipment: Domestic - Blue Ice; International - Blue Ice or Dry Ice.
Other Notes
Small volumes of anti-AQP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AQP2 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-AQP2 antibody
DB: Dot Blot, Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-AQP2 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:100 (Hoffert et al., 2008)
DB: 1:1000

Testing Data of anti-AQP2 antibody
anti-AQP2 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for AQP2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29/37
NCBI Official Full Name
aquaporin-2
NCBI Official Synonym Full Names
aquaporin 2 (collecting duct)
NCBI Official Symbol
NCBI Official Synonym Symbols
AQP-2; aquaporin-2
  [Similar Products]
NCBI Protein Information
aquaporin-2; AQP-CD; WCH-CD; aquaporin-CD; ADH water channel; collecting duct water channel protein; water channel protein for renal collecting duct
UniProt Protein Name
Aquaporin-2
UniProt Synonym Protein Names
ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
UniProt Gene Name
UniProt Synonym Gene Names
AQP-2; AQP-CD  [Similar Products]
UniProt Entry Name
AQP2_RAT
NCBI Summary for AQP2
water channel protein involved in water reabsorption and vasopressin regulation in kidney collecting duct cells; mutation is linked to nephrogenic diabetes insipidous (NDI) [RGD, Feb 2006]
UniProt Comments for AQP2
Function: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Subcellular location: Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein. Note: Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated. Ref.6

Tissue specificity: Expressed in renal collecting tubules.

Domain: Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Post-translational modification: Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent

By similarity.

Sequence similarities: Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Sequence caution: The sequence AAA41478.1 differs from that shown. Reason: Erroneous initiation.
Product References and Citations for anti-AQP2 antibody
• van Balkom BW, Savelkoul PJ, Markovich D, Hofman E, Nielsen S, van der Sluijs P, Deen PM (2002) The role of putative phosphorylation sites in the targeting and shuttling of the aquaporin 2 water channel. J Biol Chem 277(44):41473-9.
• Ford P, Rivarola V, Chara O, Blot-Chabaud M, Cluzeaud F, Farman M, Parisi M, Capurro C (2005) Volume regulation in cortical collecting duct cells: role of AQP2. Biol Cell 97(9):687-97
• Hoffert JD, Nielsen J,, Yu MJ,, Pisitikun T,, Schleicher SM,, Nielsen Knepper MA (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct.. Am J Physiol Renal Physiol 292: F691-F700.
• Iolascon A, Aglio V, Tamma G, D'Appolito M, Addabbo F, Procino G, Simonetti MC, Montini G, Gesulado L, Debler EW, Suelto M, Valenti G (2007) Characterization of two novel missense mutations in AQP2 gene causing nephrogenic diabetes insipidus. Nephron Physiol. 105(3): p33-41.
• Hoffert JD, Fenton RA, Moeller HB, Simons B, Tchapyjnikov D, McDill BW, Yu MJ, Pisitkun T, Chen F, Knepper MA. (2008) Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.

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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Organs/Tissues associated with anti-AQP2 antibody
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