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anti-COL3a1 antibody :: Rabbit anti-Rat Collagen Type III Alpha 1 (COL3a1) Polyclonal Antibody

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Catalog # MBS2006018 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.01 mg  /  $115 +1 FREE 8GB USB
  0.02 mg  /  $135 +1 FREE 8GB USB
  0.05 mg  /  $190 +1 FREE 8GB USB
  0.1 mg  /  $245 +1 FREE 8GB USB
  0.2 mg  /  $365 +1 FREE 8GB USB
  1 mg  /  $870 +1 FREE 8GB USB
Product Name

Collagen Type III Alpha 1 (COL3a1), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to Collagen Type III Alpha 1 (COL3a1)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEFELRRQAC - RGSEGSPGHP GQPGPPGPPG APGPCCGGGA AIAGVGGEKS GGFSPYYGDD PMDFKINTEE IMSSLKSVNG QIESLISPDG SRKNPARNCR DLKFCHPELK SGEYWVDPNQ GCKMDAIKVF CNMETGETCI NASPMTVPRK HWWTDAGAEK KHVWFGESMN GGFQFSYGNP DLPEDVLDVQ LAFLRLLSSR ASQNITYHCK NSIAYMDQAN GNVKKSLKLM GSNEGEFKAE GNSKFTYTVL EDGCTKHTGE WSKTVFEYQT RKAMR
3D Structure
ModBase 3D Structure for P13941
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Specificity
The antibody is a rabbit polyclonal antibody raised against COL3a1. It has been selected for its ability to recognize COL3a1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/ml (lot specific)
Immunogen
Recombinant COL3a1 (Arg1171~Arg1435) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2070524
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-COL3a1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-COL3a1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-COL3a1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-COL3a1 antibody
Western Blot: Sample: Recombinant protein.
anti-COL3a1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-COL3a1 antibody
DAB staining on fromalin fixed paraffin- embedded kidney tissue)
anti-COL3a1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-COL3a1 antibody
DAB staining on IHC-P; Samples: Rat Adrenal gland Tissue)
anti-COL3a1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for COL3a1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
138,936 Da
NCBI Official Full Name
collagen alpha-1(III) chain
NCBI Official Synonym Full Names
collagen type III alpha 1 chain
NCBI Official Symbol
Col3a1  [Similar Products]
NCBI Protein Information
collagen alpha-1(III) chain
UniProt Protein Name
Collagen alpha-1(III) chain
Protein Family
UniProt Gene Name
Col3a1  [Similar Products]
NCBI Summary for COL3a1
may be involved in craniofacial development [RGD, Feb 2006]
UniProt Comments for COL3a1
CO3A1: Collagen type III occurs in most soft connective tissues along with type I collagen. Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3); also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity. Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas. Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA). AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells. Belongs to the fibrillar collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: collagen; collagen type III trimer; extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; integrin binding; metal ion binding; platelet-derived growth factor binding; protease binding; SMAD binding

Biological Process: aging; blood vessel development; cell-matrix adhesion; cerebral cortex development; collagen fibril organization; gut development; heart development; integrin-mediated signaling pathway; negative regulation of immune response; peptide cross-linking; positive regulation of Rho protein signal transduction; response to cytokine; response to mechanical stimulus; response to radiation; skeletal system development; skin development; transforming growth factor beta receptor signaling pathway; wound healing
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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