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anti-GJA1 antibody :: Rabbit anti-Rat Connexin 43 Polyclonal Antibody

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Catalog # MBS502014
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

Connexin 43 (GJA1), Polyclonal Antibody

Full Product Name

Anti-Connexin43

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 20; NC_005119.3 (39612080..39624555). Location: 20q11
3D Structure
ModBase 3D Structure for P08050
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Specificity
Specific for the ~43k connexin43 protein.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Peptide corresponding to amino acid residues from the C-terminal region of rat connexin43.
Immunogen Information
Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Chicken, canine, finch, human, mouse non-human primate, sheep Xenopus and zebra fish
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at er368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
Preparation and Storage
Store at -20 degree C; stable for at least one year.
Other Notes
Small volumes of anti-GJA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GJA1 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-GJA1 antibody
Western Blot (WB)
Application Notes for anti-GJA1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: Not recommended

Testing Data of anti-GJA1 antibody
anti-GJA1 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for GJA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43
NCBI Official Full Name
gap junction alpha-1 protein
NCBI Official Synonym Full Names
gap junction protein, alpha 1
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1
UniProt Protein Name
Gap junction alpha-1 protein
UniProt Synonym Protein Names
Connexin-43; Cx43; Gap junction 43 kDa heart protein
UniProt Gene Name
UniProt Synonym Gene Names
Cxn-43; Cx43  [Similar Products]
UniProt Entry Name
CXA1_RAT
NCBI Summary for GJA1
gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]
UniProt Comments for GJA1
Function: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract

By similarity.

Subunit structure: A connexon is composed of a hexamer of connexins. Interacts with SGSM3. Interacts with KIAA1432/CIP150. Interacts with CNST and CSNK1D

By similarity. Interacts (via C-terminus) with TJP1. Interacts (via C-terminus) with SRC (via SH3 domain). Interacts with UBQLN4. Ref.10 Ref.12 Ref.13

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › gap junction Ref.13.

Induction: In bladder smooth muscle cells, exhibits night/day variations with low levels during the sleep phase, at circadian time (CT) 4-8 (at protein level). Expression starts to increase around CT12 and forms a plateau during the active phase (CT16-24) (at protein level). Ref.11

Post-translational modification: Contains at least one intramolecular disulfide bond.Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity

By similarity. Ref.9Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2

By similarity.S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.

Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.
Product References and Citations for anti-GJA1 antibody
• Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.
• Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.
• Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.
• Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531:132-136.

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Disclaimer
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