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anti-CREB1 antibody :: Rabbit anti-Rat CREB (Ser133) Polyclonal Antibody

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Catalog # MBS502016
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

CREB (Ser133) (CREB1), Polyclonal Antibody

Popular Item
Also Known As

Anti-Phospho-Ser133 CREB

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
341
Chromosome Location
Chromosome: 9; NC_005108.3 (71277171..71346198, complement). Location: 9q32
3D Structure
ModBase 3D Structure for P15337
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Specificity
Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus and zebra fish
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-CREB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CREB1 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-CREB1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-CREB1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:100

Testing Data of anti-CREB1 antibody
anti-CREB1 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for CREB1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
45
NCBI Official Full Name
cyclic AMP-responsive element-binding protein 1 isoform B
NCBI Official Synonym Full Names
cAMP responsive element binding protein 1
NCBI Official Symbol
Creb1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
cyclic AMP-responsive element-binding protein 1; CREB-1; Y protein; cAMP response element binding protein 1; cAMP-responsive element-binding protein 1
UniProt Protein Name
Cyclic AMP-responsive element-binding protein 1
UniProt Gene Name
Creb1  [Similar Products]
UniProt Synonym Gene Names
Creb-1; CREB-1  [Similar Products]
UniProt Entry Name
CREB1_RAT
NCBI Summary for CREB1
binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006]
UniProt Comments for CREB1
Function: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells

By similarity.

Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3

By similarity. When phosphorylated on Ser-133, binds CREBBP. Interacts with ARRB1. Interacts (phosphorylated form) with TOX3. Binds to HIPK2

By similarity. Interacts with SGK1

By similarity. Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity

By similarity.

Subcellular location: Nucleus.

Post-translational modification: Phosphorylation of Ser-133 allows CREBBP binding. Stimulated by phosphorylation. Phosphorylation of both Ser-142 and Ser-133 in the SCN regulates the activity of CREB and participate in circadian rhythm generation

By similarity. Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1. Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli

By similarity. CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity

By similarity. Ref.3 Ref.4 Ref.5Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization

By similarity.

Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP (basic-leucine zipper) domain.Contains 1 KID (kinase-inducible) domain.
Product References and Citations for anti-CREB1 antibody
• Johannessen M, Delghandi MP, Moens U (2004) What turns CREB on Cellular Signalling 16:1211-1227. Karin M, Hunter T (1995) Transcriptional control by protein phosphorylation: Signal transmission from the cell surface to the nucleus. Curr Biol 5:747-757.
• Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia ZG, Greenberg ME (2002) CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 34:221-233.
• Montminy M (1997) Transcriptional regulation by cyclic AMP. Annu Rev Biochem 66:807-822.
• Shaywitz AJ, Greenberg ME (1999) CREB: A stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu Rev Biochem 68:821-861.
• Silva AJ, Kogan JH, Frankland PW, Kida S (1998) CREB and memory. Annu Rev Neurosci 21:127-148.

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Disclaimer
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