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anti-DNM1 antibody :: Sheep anti-Rat Dynamin (Ser778) Polyclonal Antibody

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Catalog # MBS502029
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

Dynamin (Ser778) (DNM1), Polyclonal Antibody

Popular Item
Also Known As

Anti-Phospho-Ser778 Dynamin

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
851
Chromosome Location
Chromosome: 3; NC_005102.3 (16686951..16730913, complement). Location: 3p11
3D Structure
ModBase 3D Structure for P21575
Clonality
Polyclonal
Host
Sheep
Species Reactivity
Rat
Specificity
Specific for the ~95k dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by ?-phosphatase treatment.
Purity/Purification
Affinity Purified (Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser778 of rat dynamin.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Bovine, canine, chicken, human and mouse
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-DNM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DNM1 antibody
Affinity purified sheep polyclonal antibody
Applications Tested/Suitable for anti-DNM1 antibody
Western Blot (WB)
Application Notes for anti-DNM1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-DNM1 antibody
anti-DNM1 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for DNM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
95
NCBI Official Full Name
dynamin-1
NCBI Official Synonym Full Names
dynamin 1
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
dynamin-1; D100; B-dynamin; dynamin, brain
UniProt Protein Name
Dynamin-1
UniProt Synonym Protein Names
B-dynamin; D100; Dynamin, brain
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
DYN1_RAT
NCBI Summary for DNM1
mediates GTP hydrolysis; plays a role in receptor mediated endocytosis; required for agonist-induced internalization of the epidermal growth factor receptor [RGD, Feb 2006]
UniProt Comments for DNM1
Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis

By similarity.

Catalytic activity: GTP + H2O = GDP + phosphate.

Subunit structure: Interacts with CAV1 and SH3GLB1. Interacts with SNX9. Interacts with SNX33 (via SH3 domain)

By similarity. Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3

By similarity. Binds SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain). Ref.6 Ref.7 Ref.10

Subcellular location: Cytoplasm Ref.8. Cytoplasm › cytoskeleton. Note: Microtubule-associated. Isoform-specific localization. Ref.8Isoform 2: Cytoplasm Ref.8. Isoform 6: Cytoplasm Ref.8. Golgi apparatus Ref.8.

Tissue specificity: Brain-specific (peripheral sensory neurons). Ref.8

Developmental stage: Expressed in neurons after maturation.

Post-translational modification: Constitutively phosphorylated by CDK5 in nerve terminals and dephosphorylated by calcineurin when synaptic vesicle endocytosis (SVE) is stimulated by depolarization-dependent calcium influx. Ref.3 Ref.9 Ref.11

Sequence similarities: Belongs to the dynamin family.Contains 1 GED domain.Contains 1 PH domain.

Caution: Absence of tyrosine and threonine phosphorylation is demonstrated in Ref.3. However, tyrosine and threonine phosphorylation may still occur in different experimental conditions.
Product References and Citations for anti-DNM1 antibody
• Cousin MA, Tan TC, Robinson PJ (2001) Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. J Neurochem 76:105-116.
• Graham ME, O'Callaghan DW, McMahon HT, Burgoyne RD (2002) Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size. Proc Natl Acad Sci USA 99:7124-7129.
• Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003) cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5:701-710.
• Tsuboi T, Terakawa S, Scalettar BA, Fantus C, Roder J, Jeromin A (2002) Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins. J Biol Chem 277:15957-15961.
• Clayton EL, Sue N, Smillie KJ, O'Leary T, Bache N, Cheung G, Cole AR, Wyllie DJ, Sutherland C, Robinson PJ, Cousin MA. (2010) Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic vesicles. Nat Neurosci. 13(7):845-51.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated withanti-DNM1 antibody
 Reference Product  PubMed Publications
 Sh3gl2 antibody  >1 publications with DNM1 and Sh3gl2
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