• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-MAPK1/2 antibody :: Rabbit anti-Rat ERK/MAPK Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS502034
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

ERK/MAPK (MAPK1/2), Polyclonal Antibody

Full Product Name

Anti-ERK/MAPK

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 11; NC_005110.3 (91260412..91325570, complement). Location: 11q23
3D Structure
ModBase 3D Structure for P63086
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Specificity
Specific for the ~42k - 44k ERK/MAPK protein. Immunolabeling is blocked by the peptide used as antigen.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Peptide corresponding to amino acid residues from the C-terminus of rat ERK/MAPK.
Immunogen Information
Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Mouse
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with mouse tissue based on the fact that this species has 100% homology with the amino acid sequence used as antigen.
Biological Significance
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-MAPK1/2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MAPK1/2 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-MAPK1/2 antibody
Western Blot (WB)
Application Notes for anti-MAPK1/2 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-MAPK1/2 antibody
anti-MAPK1/2 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for MAPK1/2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42/44
NCBI Official Full Name
mitogen-activated protein kinase 1
NCBI Official Synonym Full Names
mitogen activated protein kinase 1
NCBI Official Symbol
Mapk1  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
mitogen-activated protein kinase 1; ERT1; ERK-2; MAPK 1; MAPK 2; p42-MAPK; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; mitogen-activated protein kinase 2; extracellular signal-regulated kinase 2; extracellular-signal-regulated kinase 2
UniProt Protein Name
Mitogen-activated protein kinase 1
UniProt Synonym Protein Names
ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2
UniProt Gene Name
Mapk1  [Similar Products]
UniProt Synonym Gene Names
Erk2; Mapk; Prkm1; MAP kinase 1; MAPK 1; ERK-2; p42-MAPK; MAP kinase 2; MAPK 2  [Similar Products]
UniProt Entry Name
MK01_RAT
NCBI Summary for MAPK1/2
kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]
UniProt Comments for MAPK1/2
Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation

By similarity. Ref.21Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity

By similarity. Ref.21

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium

By similarity.

Enzyme regulation: Phosphorylated by MAP2K1/MEK1 and MAP2K2/MEK2 on Thr-183 and Tyr-185 in response to external stimuli like insulin or NGF. Both phosphorylations are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. Phosphorylation on Ser-27 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Dephosphorylated and inactivated by DUSP3, DUSP6 and DUSP9. Inactivated by pyrimidylpyrrole inhibitors.

Subunit structure: Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with ADAM15, ARHGEF2, ARRB2, DAPK1 (via death domain), HSF4, IER3, IPO7, MKNK2, DUSP6, MORG1, NISCH, PEA15, SGK1, and isoform 1of NEK2

By similarity. Interacts (phosphorylated form) with CAV2 ('Tyr-19'-phosphorylated form); the interaction, promoted by insulin, leads to nuclear location and MAPK1 activation

By similarity. MKNK2 isoform 1binding prevents from dephosphorylation and inactivation

By similarity. Interacts with DCC. The phosphorylated form interacts with PML

By similarity. Ref.6 Ref.8 Ref.9 Ref.17 Ref.21

Subcellular location: Cytoplasm › cytoskeleton › spindle

By similarity. Nucleus

By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

By similarity. Cytoplasm

By similarity. Note: Associated with the spindle during prometaphase and metaphase

By similarity. PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention

By similarity. Phosphorylation at Ser- 244 and Ser-246 as well as autophosphorylation at Thr-188 promote nuclear localization

By similarity.

Tissue specificity: Highest levels within the nervous system, expressed in different tissues, mostly in muscle, thymus and heart.

Developmental stage: Increased expression during development.

Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Phosphorylated upon FLT3 and KIT signaling. Ligand-activated ALK induces tyrosine phosphorylation

By similarity. Dephosphorylated by PTPRJ at Tyr-185

By similarity. Autophosphorylated on threonine and tyrosine residues in vitro, which correlates with a slow and low level of activation. Phosphorylation on Ser-27 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2

By similarity. Ref.4 Ref.5ISGylated

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.
Product References and Citations for anti-MAPK1/2 antibody
• Adams JP, Sweatt JD (2002) Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 42:135-163.
• Ahn, NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 127-128:201-209.
• Johnson GL, Lapadat R (2002) Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298:1911-1912.
• Tanoue TJ, Nishida, E (2003) Molecular recognitions in the MAP kinase cascades. Cellular Signaling 15:455-462.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000