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anti-FN antibody :: Rabbit anti-Rat Fibronectin (FN) Polyclonal Antibody

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Catalog # MBS2004823 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $330 +1 FREE 8GB USB
  1 mg  /  $785 +1 FREE 8GB USB
Product Name

Fibronectin (FN), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Fibronectin (FN)

Product Gene Name
Matching Pairs
Unconjugated Antibody: Fibronectin (FN) (MBS2004823)
Biotin Conjugated Antibody: Fibronectin (FN) (MBS2006229)
Matching Pairs
Matching Pairs
Unconjugated Antibody: Fibronectin (FN) (MBS2004823)
FITC Conjugated Antibody: Fibronectin (FN) (MBS2015000)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG S- SNGEPC VLPFHYNGRT FYSCTTEGRQ DGHLWCSTTS NYEQDQKYSF CTDHAVLVQT RGGNSNGALC HFPFLYSNRN YSDCTSEGRR DNMKWCGTTQ NYDADQKFGF CPMAAHEEIC TTNEGVMYRI GDQWDKQHDL GHMMRCTCVG NGRGQWACIP YSQLRDQCIV DDITYNVNDT FHKRHEEGHM LNCTCFGQGR GRWKCDPI
3D Structure
ModBase 3D Structure for P04937
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Specificity
The antibody is a rabbit polyclonal antibody raised against FN. It has been selected for its ability to recognize FN in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
FN, Rat
Immunogen Information
Recombinant FN (Ser355~Ile558) with N-terminal His-Tag expressed in E.coli.
Accession No.
RPA037Ra01
UOM
100ug
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2035704
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-FN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-FN antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-FN antibody
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-FN antibody
Western Blot: Sample: Recombinant Fibronectin, Rat.
anti-FN antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-FN antibody
DAB staining on IHC-P; Samples: Rat Heart Tissue
anti-FN antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-FN antibody
DAB staining on IHC-P; Samples: Rat Liver Tissue.
anti-FN antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-FN antibody
DAB staining on IHC-P; Samples: Rat Kidney Tissue.
anti-FN antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-FN antibody
DAB staining on IHC-P; Samples: Rat Testis Tissue.
anti-FN antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for FN. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
259,350 Da
NCBI Official Full Name
Fibronectin
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
NCBI Official Synonym Symbols
fn-1; FIBNEC
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NCBI Protein Information
fibronectin
UniProt Protein Name
Fibronectin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for FN
extracellular matrix component; may play a role in fibrosis and tumor metastasis [RGD, Feb 2006]
UniProt Comments for FN
FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: apical plasma membrane; basal lamina; basement membrane; endoplasmic reticulum-Golgi intermediate compartment; extracellular matrix; extracellular region; extracellular space; fibrinogen complex; proteinaceous extracellular matrix

Molecular Function: chaperone binding; enzyme binding; extracellular matrix structural constituent; heparin binding; identical protein binding; integrin binding; mercury ion binding; protease activator activity; protease binding; protein binding; protein C-terminus binding; receptor binding

Biological Process: acute-phase response; angiogenesis; calcium-independent cell-matrix adhesion; cell activation; cell adhesion; cell-matrix adhesion; cell-substrate junction assembly; extracellular matrix organization; glial cell migration; integrin activation; interaction with other organism via secreted substance during symbiotic interaction; interaction with symbiont; negative regulation of apoptosis; ossification; peptide cross-linking; positive regulation of axon extension; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemotaxis; positive regulation of fibroblast proliferation; regulation of cell shape; regulation of protein amino acid phosphorylation; response to activity; response to drug; response to glucocorticoid stimulus; response to iron ion; response to ozone; response to wounding; wound healing
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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