• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-GABRA4 antibody :: Rabbit anti-Rat GABAA Receptor a 4, Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS502045
Unit / Price
Scan QR to view Datasheet
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

GABAA Receptor a 4, (GABRA4), Polyclonal Antibody

Popular Item
Full Product Name

Anti-GABAA Receptor, alpha4-Subunit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 14; NC_005113.3 (38966016..39040606). Location: 14p11
3D Structure
ModBase 3D Structure for P28471
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Specificity
Specific for the ~64k alpha4-subunit of the GABAA receptor in Western blots.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Fusion protein from the cytoplasmic loop of the alpha4-subunit of rat GABAA receptor.
Immunogen Information
Fusion protein from the cytoplasmic loop of the alpha 4 subunit
Immunogen Species
Rat
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue.
Biological Significance
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-GABRA4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GABRA4 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-GABRA4 antibody
Western Blot (WB)
Application Notes for anti-GABRA4 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-GABRA4 antibody
anti-GABRA4 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for GABRA4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
64
NCBI Official Full Name
gamma-aminobutyric acid receptor subunit alpha-4
NCBI Official Synonym Full Names
gamma-aminobutyric acid (GABA) A receptor, alpha 4
NCBI Official Symbol
Gabra4  [Similar Products]
NCBI Protein Information
gamma-aminobutyric acid receptor subunit alpha-4; GABA-A receptor alpha-4 subunit; GABA(A) receptor subunit alpha-4; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 4
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit alpha-4
UniProt Synonym Protein Names
GABA(A) receptor subunit alpha-4
UniProt Gene Name
Gabra4  [Similar Products]
UniProt Entry Name
GBRA4_RAT
NCBI Summary for GABRA4
alpha 4 subunit of the GABA-A receptor which binds gamma-aminobutyric acid; involved in mood and anxiety [RGD, Feb 2006]
UniProt Comments for GABRA4
Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily. [View classification]
Product References and Citations for anti-GABRA4 antibody
• Brandon NJ, Jovanovic JN, Colledge M, Kittler JT, Brandon JM, Scott JD, Moss SJ (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor beta-subunits. Mol Cell Neurosci 22:87-97.
• McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592.
• Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
• Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629.
• Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with anti-GABRA4 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >48 publications with GABRA4 and Brain
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000