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anti-GABRG2 antibody :: Rabbit anti-Rat GABAA Receptor g2 Polyclonal Antibody

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Catalog # MBS502055
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

GABAA Receptor g2 (GABRG2), Polyclonal Antibody

Also Known As

Anti-GABAA Receptor, gamma2-Subunit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
466
Chromosome Location
Chromosome: 10; NC_005109.3 (26934991..27024440, complement). Location: 10q21
3D Structure
ModBase 3D Structure for P18508
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Specificity
Specific for the ~46k gamma2-subunit of the GABAA receptor in Western blots
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots
Antigen
Fusion protein from the cytoplasmic loop of the gamma2-subunit of rat GABAA receptor.
Immunogen Information
Fusion protein from the cytoplasmic loop of the gamma 2 subunit
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Bovine chicken, canine, human, mouse non-human primate
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-GABRG2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GABRG2 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-GABRG2 antibody
Western Blot (WB)
Application Notes for anti-GABRG2 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-GABRG2 antibody
anti-GABRG2 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for GABRG2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
46
NCBI Official Full Name
gamma-aminobutyric acid receptor subunit gamma-2
NCBI Official Synonym Full Names
gamma-aminobutyric acid (GABA) A receptor, gamma 2
NCBI Official Symbol
Gabrg2  [Similar Products]
NCBI Protein Information
gamma-aminobutyric acid receptor subunit gamma-2; GABA(A) receptor subunit gamma-2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid A receptor, gamma 2; gamma-aminobutyric acid (GABA-A) receptor, subunit gamma 2
UniProt Protein Name
Gamma-aminobutyric acid receptor subunit gamma-2
UniProt Synonym Protein Names
GABA(A) receptor subunit gamma-2
UniProt Gene Name
Gabrg2  [Similar Products]
UniProt Entry Name
GBRG2_RAT
NCBI Summary for GABRG2
subunit of GABA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain [RGD, Feb 2006]
UniProt Comments for GABRG2
Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Post-translational modification: Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability

By similarity.

Miscellaneous: This subunit carries the benzodiazepine binding site.

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. [View classification]
Product References and Citations for anti-GABRG2 antibody
• Brandon NJ, Jovanovic JN, Colledge M, Kittler JT, Brandon JM, Scott JD, Moss SJ (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor beta-subunits. Mol Cell Neurosci 22:87-97.
• McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592.
• Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199.
• Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629.
• Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Organs/Tissues associated with anti-GABRG2 antibody
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