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anti-IFNAR1 antibody :: Rabbit anti-Rat Interferon-a Receptor, Type I, Subunit I (Ser535,539) Polyclonal Antibody

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Catalog # MBS502069
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

Interferon-a Receptor, Type I, Subunit I (Ser535,539) (IFNAR1), Polyclonal Antibody

Popular Item
Full Product Name

Anti-Phospho-Ser535,539 Interferon-alpha Receptor, Type I, Subunit I

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 21; NC_000021.9 (33324443..33359823). Location: 21q22.11
OMIM
107450
3D Structure
ModBase 3D Structure for P17181
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Specificity
Specific for IFNAR1 protein phosphorylated at Ser535,539. Note: the molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 - 130k.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser535,539 of human interferon-alpha receptor, type I, subunit I (IFNAR1).
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH
Immunogen Species
Human
Reactivity Assumed Based on 100% Sequence Homology
Bovine, canine, human, mouse, non-human primates and sheep
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human, mouse, non-human primates and sheep based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-alpha Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-IFNAR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-IFNAR1 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-IFNAR1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IFNAR1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:1000

Testing Data of anti-IFNAR1 antibody
anti-IFNAR1 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for IFNAR1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
110-130
NCBI Official Full Name
interferon alpha/beta receptor 1
NCBI Official Synonym Full Names
interferon (alpha, beta and omega) receptor 1
NCBI Official Symbol
IFNAR1  [Similar Products]
NCBI Official Synonym Symbols
AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC
  [Similar Products]
NCBI Protein Information
interferon alpha/beta receptor 1; CRF2-1; IFN-R-1; IFN-alpha/beta receptor 1; interferon-beta receptor 1; beta-type antiviral protein; alpha-type antiviral protein; type I interferon receptor 1; cytokine receptor class-II member 1; cytokine receptor family 2 member 1; interferon-alpha/beta receptor alpha chain
UniProt Protein Name
Interferon alpha/beta receptor 1
UniProt Synonym Protein Names
Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1
UniProt Gene Name
IFNAR1  [Similar Products]
UniProt Synonym Gene Names
IFNAR; IFN-R-1; IFN-alpha/beta receptor 1; CRF2-1  [Similar Products]
UniProt Entry Name
INAR1_HUMAN
NCBI Summary for IFNAR1
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq, Jul 2008]
UniProt Comments for IFNAR1
Function: Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves.

Subunit structure: Heterodimer with IFNAR2; in presence of interferon alpha and beta ligands, the heterodimer forms the type I interferon receptor. Interacts with STAT1 and STAT2; the interaction requires its phosphorylation at Tyr-466. Interacts with IFNAR2. Ref.11 Ref.12

Subcellular location: Isoform 1: Membrane; Single-pass type I membrane protein.

Tissue specificity: IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. Ref.9

Post-translational modification: Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. Ref.10Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2. Ref.14

Sequence similarities: Belongs to the type II cytokine receptor family.Contains 4 fibronectin type-III domains.

Sequence caution: The sequence AAH02590.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.
Product References and Citations for anti-IFNAR1 antibody
• Biron CA (2001) Interferons alpha and beta as immune regulators--a new look. Immunity 14:661-664.
• Kirkwood J (2002) Cancer immunotherapy: the interferon-alpha experience. Semin Oncol 29:18-26.
• Kumar KG, Tang W, Ravindranath AK, Clark WA, Croze E, Fuchs SY (2003) SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor. EMBO J 22:5480-5490.
• K. G. Suresh Kumar, John J. Krolewski, and Serge Y. Fuchs (2004) Phosphorylation and Specific Ubiquitin Acceptor Sites Are Required for Ubiquitination and Degradation of the IFNAR1 Subunit of Type I Interferon Receptor. J. Biol. Chem., Nov 2004; 279: 46614 - 46620.

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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