• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-KRT2 antibody :: Rabbit Keratin 2 (KRT2) Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS2003662 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $330 +1 FREE 8GB USB
  1 mg  /  $785 +1 FREE 8GB USB
Product Name

Keratin 2 (KRT2), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Keratin 2 (KRT2)

Matching Pairs
Matching Pairs
Unconjugated Antibody: Keratin 2 (KRT2) (MBS2003662)
APC-CY7 Conjugated Antibody: Keratin 2 (KRT2) (MBS2043050)
Matching Pairs
Unconjugated Antibody: Keratin 2 (KRT2) (MBS2003662)
PE Conjugated Antibody: Keratin 2 (KRT2) (MBS2043052)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-IAE VQSQYEIIAH KSKAESEELY HSKANEELQV TAVKHGDSLK EIKMEISELN RTIQRLQGEI SHVKKQCKGV QDSIADAEQR GEHAIKDARG KLTDLEEALQ QGRENLARLL RDYQELMNVK LALDVEIATY RKLLEGE
3D Structure
ModBase 3D Structure for Q6IG02
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Specificity
The antibody is a rabbit polyclonal antibody raised against KRT2. It has been selected for its ability to recognize KRT2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant KRT2 (Ile368~Glu507) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043054
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-KRT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-KRT2 antibody
Applications Tested/Suitable for anti-KRT2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-KRT2 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-KRT2 antibody
Western Blot: Sample: Recombinant protein.
anti-KRT2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-KRT2 antibody
DAB staining on IHC-P; Samples: Rat Cerebrum Tissue)
anti-KRT2 antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-KRT2 antibody
Knockout Validation: Lane 1: Wild-type A431 cell lysate;;Lane 2: KRT2 knockout A431 cell lysate;;Predicted MW: 69kDa ;Observed MW: 69kDa;Primary Ab: 1ug/ml Rabbit Anti-Rat KRT2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)
anti-KRT2 antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for KRT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
69,127 Da
NCBI Official Full Name
keratin, type II cytoskeletal 2 epidermal
NCBI Official Synonym Full Names
keratin 2
NCBI Official Symbol
NCBI Official Synonym Symbols
Kb2; CK 2e
  [Similar Products]
NCBI Protein Information
keratin, type II cytoskeletal 2 epidermal
UniProt Protein Name
Keratin, type II cytoskeletal 2 epidermal
UniProt Synonym Protein Names
Cytokeratin-2e; CK-2e; Epithelial keratin-2e; Keratin-2 epidermis; Keratin-2e; K2e; Type-II keratin Kb2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CK-2e; K2e  [Similar Products]
UniProt Comments for KRT2
K2: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: cornified envelope; extracellular space; keratin filament; membrane; nucleus

Molecular Function: cytoskeletal protein binding; structural constituent of epidermis; structural molecule activity

Biological Process: epidermis development; intermediate filament organization; keratinization; keratinocyte activation; keratinocyte migration; keratinocyte proliferation; peptide cross-linking
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000