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anti-KRT5 antibody :: Rabbit Keratin 5 (KRT5) Polyclonal Antibody

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Catalog # MBS2002748 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $330 +1 FREE 8GB USB
  1 mg  /  $785 +1 FREE 8GB USB
Product Name

Keratin 5 (KRT5), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to Keratin 5 (KRT5)

Matching Pairs
Unconjugated Antibody: Keratin 5 (KRT5) (MBS2002748)
Biotin Conjugated Antibody: Keratin 5 (KRT5) (MBS2001613)
Matching Pairs
Matching Pairs
Unconjugated Antibody: Keratin 5 (KRT5) (MBS2002748)
APC-CY7 Conjugated Antibody: Keratin 5 (KRT5) (MBS2043007)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-IAEVKA QYEDIANRSR TEAESWYQTK YEELQQTAGR HGDDLRNTKH EISEMNRMIQ RLRSEIDNVK KQCANLQNAI AEAEQRGELA LKDARNKLTE LEEALQKAKQ DMARLLREYQ ELMNTKLALD VEIATYRKLL EGE
3D Structure
ModBase 3D Structure for Q6P6Q2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Specificity
The antibody is a rabbit polyclonal antibody raised against KRT5. It has been selected for its ability to recognize KRT5 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant KRT5 (Ile335~Glu473) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043009
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-KRT5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-KRT5 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-KRT5 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-KRT5 antibody
Western Blot: Sample: Rat Ovary lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-KRT5 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-KRT5 antibody
Western Blot: Sample: Recombinant protein.
anti-KRT5 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-KRT5 antibody
DAB staining on fromalin fixed paraffin- embedded kidney tissue)
anti-KRT5 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-KRT5 antibody
DAB staining on IHC-P; Samples: Rat Stomach Tissue)
anti-KRT5 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for KRT5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,826 Da
NCBI Official Full Name
keratin, type II cytoskeletal 5
NCBI Official Synonym Full Names
keratin 5
NCBI Official Symbol
NCBI Official Synonym Symbols
Sak57; Krt2-5
  [Similar Products]
NCBI Protein Information
keratin, type II cytoskeletal 5
UniProt Protein Name
Keratin, type II cytoskeletal 5
UniProt Synonym Protein Names
Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CK-5; K5  [Similar Products]
NCBI Summary for KRT5
cytoskeletal intermediate filament protein; provides a cortical scaffold to primary spermatocytes [RGD, Feb 2006]
UniProt Comments for KRT5
K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: cytoplasm; intermediate filament; keratin filament; membrane; mitochondrion; nucleus; plasma membrane

Molecular Function: structural molecule activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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