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anti-MARCKS antibody :: Rabbit anti-Rat MARCKS Polyclonal Antibody

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Catalog # MBS502070
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Testing Data
Product Name

MARCKS, Polyclonal Antibody

Full Product Name


Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 20; NC_005119.3 (44693252..44698950). Location: chromosome: 20
3D Structure
ModBase 3D Structure for P30009
Species Reactivity
Specific for the ~80k MARCKS protein
Neat Serum (Unpurified neat serum.)
100 ul neat serum.
Recombinant MARCKS expressed in and purified from E. coli.
Immunogen Information
Recombinant protein expressed in and purified from E. coli
Immunogen Species
Species Reactivity Note
The antibody has been directly tested for reactivity in rat. It is expected that it will work on other mammalian tissues.
Biological Significance
Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro (Hartwig et al. 1992). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (Stumpo et al. 1995). MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-MARCKS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MARCKS antibody
Rabbit polyclonal antibody
Applications Tested/Suitable for anti-MARCKS antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-MARCKS antibody
Quality Control: Western blots performed on each lot.
WB: 1:10,000
IF: 1:500

Testing Data of anti-MARCKS antibody
anti-MARCKS antibody Testing Data image
NCBI/Uniprot data below describe general gene information for MARCKS. It may not necessarily be applicable to this product.
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
NCBI Official Full Name
Myristoylated alanine-rich C-kinase substrate
NCBI Official Synonym Full Names
myristoylated alanine rich protein kinase C substrate
NCBI Official Symbol
Marcks  [Similar Products]
NCBI Official Synonym Symbols
KINC; Macs
  [Similar Products]
NCBI Protein Information
myristoylated alanine-rich C-kinase substrate; protein kinase C substrate 80 kDa protein; Myristoylated alanine-rich protein kinase C substrate
UniProt Protein Name
Myristoylated alanine-rich C-kinase substrate
UniProt Synonym Protein Names
Protein kinase C substrate 80 kDa protein
Protein Family
UniProt Gene Name
Marcks  [Similar Products]
UniProt Synonym Gene Names
Macs; MARCKS  [Similar Products]
UniProt Entry Name
NCBI Summary for MARCKS
protein kinase C (PKC) substrate and scaffolding protein [RGD, Feb 2006]
UniProt Comments for MARCKS
Function: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Subcellular location: Cytoplasm › cytoskeleton

Probable. Membrane; Lipid-anchor

By similarity.

Tissue specificity: Highest levels found in spleen and brain. Intermediate levels seen in thymus, ovary, lung and heart. Very low levels seen in kidney, skeletal muscle and liver. Ref.3

Post-translational modification: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.

Sequence similarities: Belongs to the MARCKS family.
Product References and Citations for anti-MARCKS antibody
• Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. J Neurosci 10:1683-1698.
• Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. (1992) MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622
• Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. (1995) MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 92; 944-948.
• Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 269:9361-9367.

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