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anti-MMP12 antibody :: Rabbit anti-Rat Matrix Metalloproteinase 12 (MMP12) Polyclonal Antibody

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Catalog # MBS2005561 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $330 +1 FREE 8GB USB
  1 mg  /  $785 +1 FREE 8GB USB
Product Name

Matrix Metalloproteinase 12 (MMP12), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Matrix Metalloproteinase 12 (MMP12)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG S-V LLVSLQVSAC GAAPMNESEF AEWYLSRFFD YQGDRIPMTK TKTNRNLLEE KLQEMQQFFG LEVTGQLDTS TLKIMHTSR
3D Structure
ModBase 3D Structure for D4A7E2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Rat
Specificity
The antibody is a rabbit polyclonal antibody raised against MMP12. It has been selected for its ability to recognize MMP12 in immunohistochemical staining andwestern blotting.
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
MBS2005561-Recombinant Matrix Metalloproteinase 12 (MMP12)
Source
Polyclonal antibody preparation
Traits
Liquid
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2042012
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and stored at -20 degree C for 12 months. Avoid repeated freeze-thaw cycles.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-MMP12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MMP12 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB), IP, IF, FCM.
Application Notes for anti-MMP12 antibody
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-MMP12 antibody
Western Blot: Sample: Recombinant MMP12, Rat.
anti-MMP12 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-MMP12 antibody
DAB staining on IHC-P; Samples:Rat Liver Tissue
anti-MMP12 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-MMP12 antibody
DAB staining on IHC-P; Samples: Rat Stomach Tissue.
anti-MMP12 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-MMP12 antibody
DAB staining on IHC-P; Samples: Rat Lung Tissue.
anti-MMP12 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-MMP12 antibody
DAB staining on IHC-P; Samples: Rat Kidney Tissue.
anti-MMP12 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for MMP12. It may not necessarily be applicable to this product.
UniProt Primary Accession #
NCBI Summary for MMP12
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
UniProt Comments for MMP12
MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.65; Protease; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: proteinaceous extracellular matrix; extracellular region

Molecular Function: zinc ion binding; metalloendopeptidase activity; endopeptidase activity; calcium ion binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; positive regulation of epithelial cell proliferation involved in wound healing; proteolysis; wound healing, spreading of epidermal cells
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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