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anti-NALP3 antibody :: Goat anti-Rat NALP3 Polyclonal Antibody

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Catalog # MBS223850
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Testing Data #1
Product Name

NALP3, Polyclonal Antibody

Full Product Name

GOAT ANTI HUMAN NALP3 (C-TERMINAL)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
120100
3D Structure
ModBase 3D Structure for Q96P20
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Rat
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5mg/ml (lot specific)
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Buffer Solution
TRIS buffered saline
Antiserum Preparation
Antisera to human NALP3 were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-NALP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NALP3 antibody
Goat anti Human NALP3 antibody recognizes human NACHT, LRR and PYD domains-containing protein 3 (NALP3), also known as Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1 or Cryopyrin. NALP3 is a 1036 amino acid ~120kDa cytoplasmic protein belonging to the NLRP family, containing a single DAPIN domain, nine leucine-rich repeats and a single NACHT domain (UniProt: Q96P20). NALP3 is involved in inflammation and immune responses. NALP3 may function as an activator of NF-kappaB signalling and play a role in the regulation of inflammation and apoptosis (Choi and Ryter 2014). NALP3 is strongly expressed in polymorphonuclear cells, osteoblasts and chondrocytes, NALP3 can also be detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. Mutations in the NALP3 gene have been associated with familial cold autoinflammatory syndrome (Hoffman et al. 2001), Muckle-Wells syndrome (Dodé et al. 2002), chronic infantile neurological cutaneous and articular syndrome (Feldmann et al. 2002) and neonatal-onset multisystem inflammatory disease (Aksentijevich et al. 2002). Goat anti Human NALP3 antibody has been used successfuly for the detection of NALP3 in the eyes of rats by immunohistochemistry on formalin fixed, paraffin embedded sections (Liu et al. 2014).
Applications Tested/Suitable for anti-NALP3 antibody
ELISA (EIA), Immunohistology Paraffin*
Application Notes for anti-NALP3 antibody
ELISA: Maximum Dilution: 1/32000;
Immunohistology - Paraffin: Maximum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Testing Data #1 of anti-NALP3 antibody
Staining of formalin fixed human peripheral blood leukocytes with Goat annti Human NALP3 (MBS223850)
anti-NALP3 antibody Testing Data #1 image
NCBI/Uniprot data below describe general gene information for NALP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
115,968 Da
NCBI Official Full Name
NACHT, LRR and PYD domains-containing protein 3 isoform a
NCBI Official Synonym Full Names
NLR family, pyrin domain containing 3
NCBI Official Symbol
NLRP3  [Similar Products]
NCBI Official Synonym Symbols
AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
  [Similar Products]
NCBI Protein Information
NACHT, LRR and PYD domains-containing protein 3; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; PYRIN-containing APAF1-like protein 1; caterpiller protein 1.1; cold autoinflammatory syndrome 1 protein; cryopyrin; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
UniProt Protein Name
NACHT, LRR and PYD domains-containing protein 3
UniProt Synonym Protein Names
Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1
UniProt Gene Name
NLRP3  [Similar Products]
UniProt Synonym Gene Names
C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1  [Similar Products]
UniProt Entry Name
NALP3_HUMAN
NCBI Summary for NALP3
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]
UniProt Comments for NALP3
NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: cytoplasm; cytosol

Molecular Function: peptidoglycan binding; protein binding; ATP binding

Biological Process: caspase activation; apoptosis; negative regulation of acute inflammatory response; positive regulation of caspase activity; interleukin-1 beta production; defense response; positive regulation of interleukin-1 beta secretion; negative regulation of NF-kappaB import into nucleus; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of interleukin-1 beta secretion; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; interleukin-18 production; innate immune response; interleukin-1 secretion; detection of biotic stimulus; inflammatory response; defense response to virus

Disease: Cinca Syndrome; Familial Cold Autoinflammatory Syndrome 1; Muckle-wells Syndrome
Precautions
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Disclaimer
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