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anti-GRIN2B antibody :: Rabbit anti-Rat NMDA NR2B Subunit (Tyr1336) Polyclonal Antibody

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Catalog # MBS502090
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

NMDA NR2B Subunit (Tyr1336) (GRIN2B), Polyclonal Antibody

Popular Item
Also Known As

Anti-Phospho-Tyr1336 NMDA Receptor NR2B Subunit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1482
Chromosome Location
Chromosome: 4; NC_005103.3 (233806406..234260360, complement). Location: 4q43
3D Structure
ModBase 3D Structure for Q00960
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Specificity
Specific for ~180k NMDAR NR2B subunit protein phosphorylated at Tyr1336. Immunolabeling of the NMDAR NR2B subunit band is blocked by lambda-phosphatase treatment.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1336 of the NR2B subunit of the rat NMDA receptor.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1336 conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Human, mouse and non-human primate
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1336 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002) and ischemia may also increase the phosphorylation of this site (Takagi et al., 2003).
Preparation and Storage
Store at -20 degree C; stable for at least one year.
Other Notes
Small volumes of anti-GRIN2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-GRIN2B antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-GRIN2B antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GRIN2B antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:400

Testing Data of anti-GRIN2B antibody
anti-GRIN2B antibody Testing Data image
NCBI/Uniprot data below describe general gene information for GRIN2B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
180
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2B
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol
Grin2b  [Similar Products]
NCBI Official Synonym Symbols
GluN2B
  [Similar Products]
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2B
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B
UniProt Gene Name
Grin2b  [Similar Products]
UniProt Synonym Gene Names
GluN2B; NMDAR2B; NR2B  [Similar Products]
UniProt Entry Name
NMDE2_RAT
NCBI Summary for GRIN2B
subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]
UniProt Comments for GRIN2B
Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death

By similarity.

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Found in a complex with GRIN1 and GRIN3B. Interacts with MAGI3. Interacts with HIP1 and Neto1

By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with DAPK1

By similarity. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Post-translational modification: Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity

By similarity.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. [View classification]
Product References and Citations for anti-GRIN2B antibody
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M, Nakanishi S (1993) Molecular characterization of the family of the N-methyl- D-aspartate receptor subunits. J Biol Chem 268:2836-2843.
• Takasu, MA, Dalva, MB, Zigmond, RE, Greenberg, ME (2002) Modulation of NMDA Receptor -Dependent Calcium Influx and Gene Expression Through EphB Receptors. Science 295:491-495.
• Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358.

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