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anti-Dusp6 antibody :: Rabbit anti-Rat R MKP3 Polyclonal Antibody

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Catalog # MBS9214713
Unit / Price
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  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

R MKP3 (Dusp6), Polyclonal Antibody

Full Product Name

R MKP3 Antibody

Product Synonym Names
Dual specificity protein phosphatase 6; Mitogen-activated protein kinase phosphatase 3; MAP kinase phosphatase 3; MKP-3; Dusp6; Mkp3
Antibody/Peptide Pairs
R MKP3 peptide (MBS9228532) is used for blocking the activity of R MKP3 antibody (MBS9214713)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q64346
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Rat
Specificity
This Rat MKP3 antibody is generated from rabbits immunized with a 6xHIS-tagged recombinant protein encoding aa 1~381 of rat MKP3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (lot specific)
Antigen Type
Recombinant Protein
Antigen Source
RAT
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-Dusp6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Dusp6 antibody
Dual specificity protein tyrosine phosphatases (dsPTPs) are a subfamily of protein tyrosine phosphatases implicated in the regulation of mitogen-activated protein kinase (MAPK). MKP-1 (also known as CL100, 3CH134, Erp, and hVH-1) exemplifies a class of dual-specificity phosphatase able to reverse the activation of MAP kinases by dephosphorylating critical tyrosine and threonine residues. MKP-3 is 36% identical to MKP-1 and it blocks both the phosphorylation and enzymatic activation of ERK2 by mitogens. MKP-3 mRNA is expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis
Product Categories/Family for anti-Dusp6 antibody
Applications Tested/Suitable for anti-Dusp6 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes for anti-Dusp6 antibody
WB~~1:1000

Western Blot (WB) of anti-Dusp6 antibody
Western blot analysis of MKP3 (arrow) using rabbit polyclonal MKP3-His6 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MKP3 gene.
anti-Dusp6 antibody Western Blot (WB) (WB) image
Flow Cytometry (FC/FACS) of anti-Dusp6 antibody
Rat MKP3 Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-Dusp6 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
NCBI/Uniprot data below describe general gene information for Dusp6. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
42319
NCBI Official Full Name
dual specificity protein phosphatase 6
NCBI Official Synonym Full Names
dual specificity phosphatase 6
NCBI Official Symbol
Dusp6  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
dual specificity protein phosphatase 6
UniProt Protein Name
Dual specificity protein phosphatase 6
UniProt Synonym Protein Names
Mitogen-activated protein kinase phosphatase 3; MAP kinase phosphatase 3; MKP-3
UniProt Gene Name
Dusp6  [Similar Products]
UniProt Synonym Gene Names
Mkp3; MAP kinase phosphatase 3; MKP-3  [Similar Products]
UniProt Entry Name
DUS6_RAT
NCBI Summary for Dusp6
protein tyrosine phosphatase involved in regulation of mitogen-activated protein kinase (MAPK) [RGD, Feb 2006]
UniProt Comments for Dusp6
MKP-3: a non-receptor, dual-specificity phosphoprotein phosphatase. Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This protein inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.16; EC 3.1.3.48; Protein phosphatase, dual-specificity

Cellular Component: cytoplasm; cytosol

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity; phosphoprotein phosphatase activity

Biological Process: response to organic substance; response to drug; response to nitrosative stress; negative regulation of protein amino acid phosphorylation; dorsal/ventral pattern formation; positive regulation of apoptosis; regulation of fibroblast growth factor receptor signaling pathway; regulation of endodermal cell fate specification; protein amino acid dephosphorylation; cell differentiation; response to organic cyclic substance; inactivation of MAPK activity
Product References and Citations for anti-Dusp6 antibody
Muda,M., et al. J. Biol. Chem. 271 (8), 4319-4326 (1996)
Mourey,R.J., et al. J. Biol. Chem. 271 (7), 3795-3802 (1996)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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