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anti-SYT1 antibody :: Rabbit anti-Rat Synaptotagmin (Ser309) Polyclonal Antibody

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Catalog # MBS502122
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

Synaptotagmin (Ser309) (SYT1), Polyclonal Antibody

Also Known As

Anti-Phospho-Ser309 Synaptotagmin

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
421
Chromosome Location
Chromosome: 7; NC_005106.3 (50097034..50289950, complement). Location: 7q21
3D Structure
ModBase 3D Structure for P21707
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Specificity
Specific for the ~60k-62k synaptotagmin protein phosphorylated at Ser309.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser309 of synaptotagmin.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Bovine, canine, chicken, human, mouse, non-human primate and zebra fish
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-SYT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SYT1 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-SYT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SYT1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:400

Testing Data of anti-SYT1 antibody
anti-SYT1 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for SYT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60/62
NCBI Official Full Name
synaptotagmin-1
NCBI Official Synonym Full Names
synaptotagmin I
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
synaptotagmin-1; sytI; synaptotagmin 1
UniProt Protein Name
Synaptotagmin-1
UniProt Synonym Protein Names
Synaptotagmin I; SytI; p65
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
SYT1_RAT
NCBI Summary for SYT1
integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006]
UniProt Comments for SYT1
Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2.

Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.

Subunit structure: Homotetramer

Probable. Interacts with SCAMP5 and STON2. Forms a complex with SV2B, syntaxin 1 and SNAP25

By similarity. Interacts with RIMS1. Interacts with SV2A, SV2B and SV2C. Ref.5 Ref.6 Ref.8

Subcellular location: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass membrane protein. Cytoplasm. Note: Synaptic vesicles and chromaffin granules.

Tissue specificity: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues.

Domain: The first C2 domain mediates Ca2+-dependent phospholipid binding. Ref.5The second C2 domain mediates interaction with SV2A and probably with STN2. Ref.5

Sequence similarities: Belongs to the synaptotagmin family.Contains 2 C2 domains.
Product References and Citations for anti-SYT1 antibody
• Fernandez-Chacon R, Konigstorfer A, Gerber SH, Garcia J, Matos MF, Stevens CF, Brose N, Rizo J, Rosenmund C, Sudhof TC (2001) Synaptotagmin I functions as a calcium regulator of release probability. Nature (London) 410:41-49.
• Hilfiker S, Pieribone VA, Nordstedt C, Greengard P, Czernik AJ (1999) Regulation of synaptotagmin I phosphorylation by multiple protein kinases. J Neurochem 73:921-932.
• Lee BH, Min X, Heise CJ, Xu BE, Chen S, Shu H, Luby-Phelps K, Goldsmith EJ, Cobb MH (2004) WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Mol Cell 15:741-751.
• Poskanzer KE, Marek KW, Sweeney ST, Davis GW (2003) Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature (London) 426:559-563.
• Wang CT, Lu JC, Bai JH, Chang PY, Martin TFJ, Chapman ER, Jackson MB (2003) Different domains of synaptotagmin control the choice between kiss-and-run and full fusion. Nature (London) 424:943-947.

Precautions
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Disclaimer
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