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5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG Secondary Antibody

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Catalog # MBS176695
Unit / Price
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  1 mL  /  $180 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG, Secondary Antibody

Popular Item
Full Product Name

5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Specificity
This colloidal gold conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse, bovine IgG.
Form/Format
Concentrated, Liquid
Immunogen
Rabbit IgG (whole molecule).
Contents
0.1 mg of colloidal gold conjugated specific antibody. the diameter of colloidal gold is 5±1 nm, 0.01 M PBS, 0.01 % Thimerosal, 50% glycerol
Preparation of Colloidal Gold
5 nm Colloidal Gold - Add 0.05% trisodium citrate and 0.05% Vitamin C into boiled 0.01 % chloroauric acid which was reduced to gold granule solution through redox reaction. Conjugate colloidal gold to antibody after mensurating the optimal data. And through overspeed centrifugation and gradient delamination to density, the colloidal gold conjugated reagents were produced.
Preparation of Diluent Buffer
Add reagent grade BSA into 0.01 M PBS (PH7.2-7.6) or TBS buffer and make BSA at a concentration of 1%. Use the above diluent buffer to dilute.
Preparation of IGSS (lmmunoGold Silver Staining)
Add citric acid 2.35g, trisodium citrate 2.55g, glutin 1g and hydroquinone 1.7g into 100 ml of distilled water and mix evenly. Before usage, add silver nitrate 50mg into it. Then, add this mixed solution into tissue at last and colorate for 10-20 minutes in dark. Wash with water to terminate reaction.
Preparation and Storage
At 4 degree C for one year.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of 5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Description: This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins and bovine serum proteins to ensure minimal cross reactivity in tissue or cell preparations.
Electron Microscopy (EM)
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
EM:1:20-50
IHC-P: 1:50-100
IHC-F: 1:50-100
ICC: 1:50-100

Optimal working dilutions must be determined by end users.

Applications: Electron Microscopy
Sample: Oyster Visceral Mass
Detection: 5nm Colloidal Gold
"To validate the specificity of the anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) ABIN3042038, 8um paraffin sections of oyster's visceral mass were observed in this study. We compared the level of silver aggregating ability in the presence and absence of ABIN3042038, using commercially available silver enhancement method and SEM-EBSD imaging. We found that the test samples treated with anti-Rabbit IgG (Whole Molecule) Antibody (Colloidal Gold (5nm)) showed distinctive silver aggregates while the primary rabbit anti-SNAP antibody ABIN1573927 was present. These aggregates were not observable in the negative control."
5 nm Colloidal Gold Conjugated Goat Anti-rabbit IgG secondary antibody Immunohistochemistry (IHC) (IHC) image
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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