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Polyclonal Antibody

What is Polyclonal Antibody?

Polyclonal antibodies can be used in a wide range of antibody-based assays. They are produced by injecting an immunogen into a host species (usually a rabbit). The serum can then be collected and purified. Purification can be done using physicochemical fractionation, class-specific affinity purification or antigen-affinity purification. Compared to monoclonal antibodies, polyclonal antibodies will recognize multiple epitopes which can offer many advantages. Polyclonal antibodies can be used to amplify signals from target proteins with low expression levels and can often give better results in ChIP (chromatin immunoprecipitation) experiments and similar applications. They are also advantageous for detecting denatured proteins because polyclonal antibodies are more tolerant of minor changes in the antigen.

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