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PON1 elisa kit :: Human Paraoxonase 1 (PON1) ELISA Kit

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Catalog # MBS2022720 (SPECIAL PROMOTION: Get FREE Starbucks gift card ($5 for 48-Wells-Kit or $10 for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  24-Strip-Wells  /  $255 +1 FREE 8GB USB
  48-Strip-Wells  /  $455 +1 FREE 8GB USB
  96-Strip-Wells  /  $610 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,470 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $4,445 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Paraoxonase 1 (PON1), ELISA Kit

Popular Item
Also Known As

Paraoxonase 1 (PON1) ELISA Kit

Product Synonym Names
ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
6
OMIM
168820
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of PON1. No significant cross-reactivity or interference between PON1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
3.12-200ng/mL
Sensitivity
1.27ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PON1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PON1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PON1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPON1purchase
MBS2022720 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Paraoxonase 1 (PON1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PON1. The ELISA analytical biochemical technique of the MBS2022720 kit is based on PON1 antibody-PON1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PON1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PON1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PON1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PON1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PON1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PON1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PON1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for PON1 elisa kit

Typical Testing Data/Standard Curve (for reference only) of PON1 elisa kit
PON1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2022720. Click to request current manual
NCBI/Uniprot data below describe general gene information for PON1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
39,731 Da
NCBI Official Full Name
paraoxonase 1, partial
NCBI Official Synonym Full Names
paraoxonase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
ESA; PON; MVCD5
  [Similar Products]
NCBI Protein Information
serum paraoxonase/arylesterase 1
UniProt Protein Name
Serum paraoxonase/arylesterase 1
UniProt Synonym Protein Names
Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1
UniProt Gene Name
UniProt Synonym Gene Names
PON; PON 1; A-esterase 1  [Similar Products]
UniProt Entry Name
PON1_HUMAN
NCBI Summary for PON1
The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]
UniProt Comments for PON1
PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family.

Protein type: Motility/polarity/chemotaxis; EC 3.1.1.2; Lipid-binding; EC 3.1.8.1; Phosphatase (non-protein); Hydrolase; Secreted; Secreted, signal peptide; EC 3.1.1.81

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: extracellular space; intracellular membrane-bound organelle; extracellular region

Molecular Function: protein homodimerization activity; arylesterase activity; phospholipid binding; calcium ion binding; aryldialkylphosphatase activity

Biological Process: response to external stimulus; response to nutrient levels; dephosphorylation; response to toxin; organophosphate catabolic process; positive regulation of transporter activity; carboxylic acid catabolic process; positive regulation of binding; aromatic compound catabolic process; phosphatidylcholine metabolic process

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with PON1 elisa kit
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