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Pon3 elisa kit :: Rat Serum paraoxonase/lactonase 3 ELISA Kit

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Catalog # MBS2881619
Unit / Price
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  48-Strip-Wells  /  $400 +1 FREE 8GB USB
  96-Strip-Wells  /  $530 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,305 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $4,250 +5 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Serum paraoxonase/lactonase 3 (Pon3), ELISA Kit

Popular Item
Full Product Name

Rat Serum paraoxonase/lactonase 3 ELISA Kit

Product Synonym Names
Serum paraoxonase/lactonase 3; Pon3; 3.1.1.2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
3D Structure
ModBase 3D Structure for Q68FP2
Species Reactivity
Specificity
Recombinant and natural Rat Serum paraoxonase/lactonase 3
Assay Type
Sandwich
Detection Range
0.312-20 ng/mL
Sensitivity
0.162 ng/mL
Intra-Assay CV
<=4.9%
Inter-Assay CV
<=6.2%
Recovery
86%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of Pon3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Pon3purchase
MBS2881619 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Serum paraoxonase/lactonase 3 (Pon3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Pon3. The ELISA analytical biochemical technique of the MBS2881619 kit is based on Pon3 antibody-Pon3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Pon3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Pon3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
Pon3 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of Pon3 elisa kit
Pon3 elisa kit Typical Testing Data/Standard Curve (for reference only) image
NCBI/Uniprot data below describe general gene information for Pon3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
39,458 Da
NCBI Official Full Name
serum paraoxonase/lactonase 3
NCBI Official Synonym Full Names
paraoxonase 3
NCBI Official Symbol
NCBI Protein Information
serum paraoxonase/lactonase 3
UniProt Protein Name
Serum paraoxonase/lactonase 3
UniProt Gene Name
UniProt Entry Name
PON3_RAT
NCBI Summary for Pon3
microsomal paraoxonase that has esterase activity on a broad range of substrates [RGD, Feb 2006]
UniProt Comments for Pon3
Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with Pon3 elisa kit
Pathways associated with Pon3 elisa kit
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