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PP elisa kit :: Human PP ELISA Kit

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Catalog # MBS2511449
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PP, ELISA Kit

Popular Item
Full Product Name

Human PP (Pepsin) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
167780
3D Structure
ModBase 3D Structure for P01298
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PP. No significant cross-reactivity or interference between Human PP and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PP and all the analogues, therefore, cross reaction may still exist.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
62.5-4000pg/mL
Sensitivity
Min: 37.5pg/mL; Max: 4000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPPpurchase
MBS2511449 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PP. The ELISA analytical biochemical technique of the MBS2511449 kit is based on PP antibody-PP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PP elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PP in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PP. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PP and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PP. You can calculate the concentration of Human PP in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PP elisa kit
PP elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2511449. Click to request current manual
NCBI/Uniprot data below describe general gene information for PP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
10,445 Da
NCBI Official Full Name
pancreatic prohormone preproprotein
NCBI Official Synonym Full Names
pancreatic polypeptide
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
pancreatic prohormone; PP; pancreatic polypeptide Y; prepro-PP (prepropancreatic polypeptide)
UniProt Protein Name
Pancreatic prohormone
UniProt Synonym Protein Names
Pancreatic polypeptide; PP; INN: ObinepitideCleaved into the following 2 chains:Pancreatic hormone; PH; Pancreatic icosapeptide; PI
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PNP; PP; PH; PI  [Similar Products]
UniProt Entry Name
PAHO_HUMAN
NCBI Summary for PP
This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. [provided by RefSeq, Jul 2008]
UniProt Comments for PP
PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; extracellular region

Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding; hormone activity; receptor binding

Biological Process: cell-cell signaling; neuropeptide signaling pathway; digestion; regulation of appetite; protein secretion; feeding behavior
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with PP elisa kit
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