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PPAR-alpha elisa kit :: Human PPAR-alpha ELISA Kit

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Catalog # MBS2506110
Unit / Price
  48-Strip-Wells  /  $410 +1 FREE 8GB USB
  96-Strip-Wells  /  $490 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,040 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,590 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PPAR-alpha, ELISA Kit

Popular Item
Full Product Name

Human PPAR-alpha (Peroxisome Proliferator Activated Receptor Alpha) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2506110 COA
OMIM
170998
3D Structure
ModBase 3D Structure for Q07869
Species Reactivity
Specificity
This kit recognizes Human PPAR-? in samples. No Significant cross-reactivity or interference between Human PPAR-? and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PPAR-? were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PPAR-? were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PPAR-alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPPAR-alphapurchase
MBS2506110 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PPAR-alpha, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PPAR-alpha. The ELISA analytical biochemical technique of the MBS2506110 kit is based on PPAR-alpha antibody-PPAR-alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPAR-alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPAR-alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PPAR-alpha elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PPAR-? concentrations in serum, plasma and other biological fluids.

Typical Testing Data/Standard Curve (for reference only) of PPAR-alpha elisa kit
PPAR-alpha elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2506110. Click to request current manual
NCBI/Uniprot data below describe general gene information for PPAR-alpha. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
18,942 Da
NCBI Official Full Name
peroxisome proliferator-activated receptor alpha
NCBI Official Synonym Full Names
peroxisome proliferator-activated receptor alpha
NCBI Official Symbol
PPARA  [Similar Products]
NCBI Official Synonym Symbols
PPAR; NR1C1; hPPAR; PPARalpha
  [Similar Products]
NCBI Protein Information
peroxisome proliferator-activated receptor alpha; PPAR-alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3
UniProt Protein Name
Peroxisome proliferator-activated receptor alpha
UniProt Synonym Protein Names
Nuclear receptor subfamily 1 group C member 1
UniProt Gene Name
PPARA  [Similar Products]
UniProt Synonym Gene Names
NR1C1; PPAR; PPAR-alpha  [Similar Products]
UniProt Entry Name
PPARA_HUMAN
NCBI Summary for PPAR-alpha
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for PPAR-alpha
PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; protein binding; NFAT protein binding; DNA binding; ubiquitin conjugating enzyme binding; zinc ion binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; drug binding; lipid binding; transcription factor activity; phosphatase binding

Biological Process: circadian rhythm; epidermis development; wound healing; heart development; positive regulation of transcription, DNA-dependent; behavioral response to nicotine; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; fatty acid metabolic process; negative regulation of appetite; response to insulin stimulus; positive regulation of gluconeogenesis; circadian regulation of gene expression; negative regulation of blood pressure; negative regulation of glycolysis; negative regulation of protein binding; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; lipoprotein metabolic process; regulation of circadian rhythm; positive regulation of fatty acid beta-oxidation; positive regulation of fatty acid oxidation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; gene expression; steroid hormone mediated signaling; lipid metabolic process; fatty acid transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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