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Add To Compare List PPARG elisa kit :: peroxisome proliferator-activated receptor gamma ELISA Kit Catalog # MBS703940 Unit / Price 24-Strip-Wells (LIMIT 1) / $285 +1 FREE 8GB USB 48-Strip-Wells / $650 +1 FREE 8GB USB 96-Strip-Wells / $930 +1 FREE 8GB USB 5x96-Strip-Wells / $3,370 +4 FREE 8GB USB 10x96-Strip-Wells / $6,395 +7 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name peroxisome proliferator-activated receptor gamma (PPARG), ELISA Kit Popular Item Full Product Name Rat Peroxisome Proliferator-activated receptor gamma, PPAR-gamma ELISA Kit Product Synonym Names Rat Peroxisome Proliferator-activated receptor gamma; PPAR-gamma ELISA Kit; CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARgamma; OTTHUMP00000185030; OTTHUMP00000185033; PPAR gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferative activated receptor gamma; peroxi; peroxisome proliferator-activated receptor gamma Product Gene Name PPARG elisa kit[Similar Products] Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual MBS703940 Testing Data Testing Data PDF 3D Structure ModBase 3D Structure for O19052 Species Reactivity Rat Specificity This assay has high sensitivity and excellent specificity for detection of Rat PPARG. No significant cross-reactivity or interference between Rat PPARG and analogues was observed. Intra-assay Precision Intra-assay Precision (Precision within an assay): CV% is less than 8%Three samples of known concentration were tested twenty times on one plate to assess.Inter-assay Precision (Precision between assays): CV% is less than 10%Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength 450 nm Sample Volume 50-100ul Protein Biological Process 1 Transcription/Transcription regulation Protein Biological Process 3 Transcription Preparation and Storage Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions. ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of PPARG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. Searchable Terms for PPARG purchase MBS703940 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the peroxisome proliferator-activated receptor gamma (PPARG) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PPARG. The ELISA analytical biochemical technique of the MBS703940 kit is based on PPARG antibody-PPARG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPARG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPARG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). TOP Related Product Information for PPARG elisa kit Introduction: Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. The PPAR gamma 2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with RXR alpha. Defects in PPAR gamma can lead to type 2 insulin-resistant diabetes and hyptertension and could play a role in the genetic predisposition to obesity.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPAR-gamma. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PPAR-gamma and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PPAR-gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PPAR-gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve. Typical Testing Data/Standard Curve (for reference only) of PPARG elisa kit TOP Sample Manual Insert of MBS703940. NCBI/Uniprot data below describe general gene information for PPARG. It may not necessarily be applicable to this product. NCBI GI # 3024414 NCBI Accession # O19052.1 [Other Products] UniProt Primary Accession # O19052 [Other Products] Molecular Weight 54,365 Da TOP NCBI Official Full Name Peroxisome proliferator-activated receptor gamma UniProt Protein Name Peroxisome proliferator-activated receptor gamma UniProt Synonym Protein Names Nuclear receptor subfamily 1 group C member 3 Protein Family Peroxisome proliferator-activated receptor UniProt Gene Name PPARG [Similar Products] UniProt Synonym Gene Names NR1C3; PPAR-gamma [Similar Products] UniProt Entry Name PPARG_RABIT TOP TOP UniProt Comments for PPARG Function: Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses Enzyme regulation: PDPK1 activates its transcriptional activity independently of its kinase activity Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP Products associated with PPARG elisa kit Reference Product PubMed Publications VEGF elisa kit >160 publications with PPARG and VEGF CCL2 elisa kit >144 publications with PPARG and CCL2 PTEN elisa kit >80 publications with PPARG and PTEN NCOA1 elisa kit >27 publications with PPARG and NCOA1 MED1 elisa kit >21 publications with PPARG and MED1 CDK5 elisa kit >17 publications with PPARG and CDK5 RXRgamma elisa kit >12 publications with PPARG and RXRgamma RXRbeta elisa kit >9 publications with PPARG and RXRbeta Pathways associated with PPARG elisa kit Products by Pathway Pathway Diagram Developmental Biology Pathway antibodies Developmental Biology Pathway Diagram Gene Expression Pathway antibodies Gene Expression Pathway Diagram Generic Transcription Pathway antibodies Generic Transcription Pathway Diagram Huntington's Disease Pathway antibodies Huntington's Disease Pathway Diagram Huntington's Disease Pathway antibodies Huntington's Disease Pathway Diagram Nuclear Receptor Transcription Pathway antibodies Nuclear Receptor Transcription Pathway Diagram Nuclear Receptors Pathway antibodies Nuclear Receptors Pathway Diagram Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram Osteoclast Differentiation Pathway antibodies Osteoclast Differentiation Pathway Diagram Osteoclast Differentiation Pathway antibodies Osteoclast Differentiation Pathway Diagram Diseases associated with PPARG elisa kit Disease Name Pubmed Publications Metabolic Diseases Antibodies >2092 publications with PPARG and Metabolic Diseases Inflammation Antibodies >1367 publications with PPARG and Inflammation Insulin Resistance Antibodies >1351 publications with PPARG and Insulin Resistance Diabetes Mellitus Antibodies >1350 publications with PPARG and Diabetes Mellitus Obesity Antibodies >1278 publications with PPARG and Obesity Cardiovascular Diseases Antibodies >1175 publications with PPARG and Cardiovascular Diseases Neoplasms Antibodies >1139 publications with PPARG and Neoplasms Diabetes Mellitus, Type 2 Antibodies >934 publications with PPARG and Diabetes Mellitus, Type 2 Necrosis Antibodies >722 publications with PPARG and Necrosis Atherosclerosis Antibodies >600 publications with PPARG and Atherosclerosis Organs/Tissues associated with PPARG elisa kit Organ/Tissue Name Pubmed Publications Adipose Tissue Antibodies >1321 publications with PPARG and Adipose Tissue Muscle Antibodies >1134 publications with PPARG and Muscle Brain Antibodies >445 publications with PPARG and Brain Kidney Antibodies >425 publications with PPARG and Kidney Intestine Antibodies >196 publications with PPARG and Intestine Skin Antibodies >144 publications with PPARG and Skin Whole Body Antibodies >113 publications with PPARG and Whole Body Reticulum Antibodies >61 publications with PPARG and Reticulum Uterus Antibodies >42 publications with PPARG and Uterus Mammary Gland Antibodies >25 publications with PPARG and Mammary Gland TOP © 2006-24 MyBioSource.com. 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