• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PPIF recombinant protein :: Cyclophilin F Recombinant Protein

Scan QR to view Datasheet
Catalog # MBS203258
Unit / Price
  0.1 mg  /  $255 +1 FREE 8GB USB
  0.5 mg  /  $535 +1 FREE 8GB USB
SDS-Page
Product Name

Cyclophilin F (PPIF), Recombinant Protein

Popular Item
Also Known As

Cyclophilin F (PPIF), 30-207aa, Human,His tag, E Coli (Bioactivity Validated)

Product Synonym Names
Peptidylprolyl isomerase F; Peptidylprolyl isomerase F; CYP3; Cyp-D; PPIF; Peptidylprolyl isomerase F Cyclophilin 3; Cyclophilin D; Cyclophilin F; Cyp D; CYP3; CypD; hCyP3; Peptidylprolyl isomerase F (cyclophilin F); Peptidyl prolyl cis trans isomerase; mitochondral; Peptidylprolyl isomerase F; PPIase; Rotamase.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
207
Sequence
MGSSHHHHHH SSGLVPRGSH MCSKGSGDPS SSSSSGNPLV YLDVDANGKP LGRVVLELKA DVVPKTAENF RALCTGEKGF GYKGSTFHRV IPSFMCQAGD FTNHNGTGGK SIYGSRFPDE NFTLKHVGPG VLSMANAGPN TNGSQFFICT IKTDWLDGKH VVFGHVKEGM DVVKKIESFG SKSGRTSKKI VITDCGQLS
OMIM
M80254 mRNA
3D Structure
ModBase 3D Structure for P30405
Host
E Coli
Purity/Purification
> 95% by SDS PAGE
Form/Format
Liquid in 20mM Tris pH 7.5, 1mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of PPIF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PPIF recombinant protein
Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. Recombinant PPIF protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PPIF recombinant protein
Applications Tested/Suitable for PPIF recombinant protein
SDS-PAGE

NCBI/Uniprot data below describe general gene information for PPIF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21.1 kDa (199aa), confirmed by MALDI-TOF. [Similar Products]
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase F, mitochondrial
NCBI Official Synonym Full Names
peptidylprolyl isomerase F
NCBI Official Symbol
NCBI Official Synonym Symbols
CYP3; CypD; CyP-M; Cyp-D
  [Similar Products]
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Synonym Protein Names
Cyclophilin D; CyP-D; CypD; Cyclophilin F; Mitochondrial cyclophilin; CyP-M; Rotamase F
UniProt Gene Name
UniProt Synonym Gene Names
CYP3; PPIase F; CyP-D; CypD; CyP-M  [Similar Products]
NCBI Summary for PPIF
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
UniProt Comments for PPIF
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F1F0 ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.
Product References and Citations for PPIF recombinant protein
Bergsma DJ., et al. (1991) J Biol Chem. 266 (34):23204-14.; Bowles KR., et al. (2000) Genomics 67 (2):109-27.

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withPPIF recombinant protein
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000