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PPP1R2 sirna

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Catalog # MBS8215256
Unit / Price
  15 nmol  /  $365 +1 FREE 8GB USB
  30 nmol  /  $515 +1 FREE 8GB USB
PPP1R2 sirna
Product Name

PPP1R2, siRNA

Also Known As

PPP1R2 siRNA (Rat)

Product Synonym Names
IPP2; Protein phosphatase inhibitor 2; IPP-2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
205
3D Structure
ModBase 3D Structure for P50411
Host
Synthetic
Species Reactivity
Rat
Specificity
PPP1R2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PPP1R2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PPP1R2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PPP1R2 sirna
siRNA to inhibit PPP1R2 expression using RNA interference
Applications Tested/Suitable for PPP1R2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PPP1R2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,071 Da
NCBI Official Full Name
protein phosphatase inhibitor 2
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory (inhibitor) subunit 2
NCBI Official Symbol
Ppp1r2  [Similar Products]
NCBI Official Synonym Symbols
IPP-2
  [Similar Products]
NCBI Protein Information
protein phosphatase inhibitor 2
UniProt Protein Name
Protein phosphatase inhibitor 2
UniProt Gene Name
Ppp1r2  [Similar Products]
UniProt Synonym Gene Names
Ipp2; IPP-2  [Similar Products]
UniProt Entry Name
IPP2_RAT
NCBI Summary for PPP1R2
may play a role in the regulation of neurogenesis [RGD, Feb 2006]
UniProt Comments for PPP1R2
PPP1R2: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.

Protein type: Inhibitor; Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Molecular Function: protein phosphatase inhibitor activity

Biological Process: glycogen metabolic process; negative regulation of catalytic activity; regulation of signal transduction; regulation of phosphoprotein phosphatase activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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