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PRAME elisa kit :: Human Melanoma antigen preferentially expressed in tumors ELISA Kit

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Catalog # MBS2886190
Unit / Price
  48-Strip-Wells  /  $355 +1 FREE 8GB USB
  96-Strip-Wells  /  $475 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,030 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,700 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

Melanoma antigen preferentially expressed in tumors (PRAME), ELISA Kit

Popular Item
Also Known As

Human Melanoma antigen preferentially expressed in tumors ELISA Kit

Product Synonym Names
Melanoma antigen preferentially expressed in tumors; Opa-interacting protein 4; OIP-4; Preferentially expressed antigen of melanoma; PRAME; MAPE; OIP4
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS2886190 Testing Data
Sequence Length
509
OMIM
606021
3D Structure
ModBase 3D Structure for P78395
Species Reactivity
Specificity
Recombinant and natural Human Melanoma antigen
Samples
serum, plasma, tissue homogenates?cell culture supernates or other biological fluids
Detection Range
0.156-10 ng/mL
Sensitivity
<0.056 ng/mL
Intra-assay Precision
Intra-Assay CV: ?4.9%
Inter-assay Precision
Inter-Assay CV: ?8.8%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PRAME elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPRAMEpurchase
MBS2886190 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Melanoma antigen preferentially expressed in tumors (PRAME) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRAME. The ELISA analytical biochemical technique of the MBS2886190 kit is based on PRAME antibody-PRAME antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRAME antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRAME. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PRAME elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates?cell culture supernates or other biological fluids.

Typical Testing Data/Standard Curve (for reference only) of PRAME elisa kit
PRAME elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2886190. Click to request current manual
NCBI/Uniprot data below describe general gene information for PRAME. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,890 Da
NCBI Official Full Name
melanoma antigen preferentially expressed in tumors isoform a
NCBI Official Synonym Full Names
preferentially expressed antigen in melanoma
NCBI Official Symbol
PRAME  [Similar Products]
NCBI Official Synonym Symbols
MAPE; OIP4; CT130; OIP-4
  [Similar Products]
NCBI Protein Information
melanoma antigen preferentially expressed in tumors
UniProt Protein Name
Melanoma antigen preferentially expressed in tumors
UniProt Synonym Protein Names
Opa-interacting protein 4; OIP-4; Preferentially expressed antigen of melanoma
Protein Family
UniProt Gene Name
PRAME  [Similar Products]
UniProt Synonym Gene Names
MAPE; OIP4; OIP-4  [Similar Products]
UniProt Entry Name
PRAME_HUMAN
NCBI Summary for PRAME
This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
UniProt Comments for PRAME
PRAME: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Belongs to the PRAME family.

Protein type: Cancer Testis Antigen (CTA); Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 22q11.22

Cellular Component: nucleus; plasma membrane

Molecular Function: protein binding; retinoic acid receptor binding

Biological Process: apoptosis; cell differentiation; negative regulation of apoptosis; negative regulation of cell differentiation; negative regulation of retinoic acid receptor signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; regulation of growth; transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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