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PRAP1 elisa kit :: Human Proline-rich acidic protein 1 ELISA Kit

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Catalog # MBS765465
Unit / Price
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  48-Strip-Wells  /  $300 +1 FREE 8GB USB
  96-Strip-Wells  /  $415 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,825 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,425 +4 FREE 8GB USB
Product Name

Proline-rich acidic protein 1 (PRAP1), ELISA Kit

Popular Item
Full Product Name

Human Proline-rich acidic protein 1 ELISA Kit

Product Synonym Names
PRAP1 (Proline-rich acidic protein 1)/Uterine-specific proline-rich acidic protein/Uterine-specific proline-rich acidic protein
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS765465 COA
OMIM
609776
Species Reactivity
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
78-5000pg/ml
Sensitivity
46.9pg/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PRAP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PRAP1purchase
MBS765465 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proline-rich acidic protein 1 (PRAP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRAP1. The ELISA analytical biochemical technique of the MBS765465 kit is based on PRAP1 antibody-PRAP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRAP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRAP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PRAP1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PRAP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PRAP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PRAP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PRAP1 can be calculated.

Typical Testing Data of PRAP1 elisa kit
PRAP1 elisa kit Typical Testing Data image
Typical Standard Curve of PRAP1 elisa kit
PRAP1 elisa kit Typical Standard Curve image
Sample Manual Insert of MBS765465. Click to request current manual
NCBI/Uniprot data below describe general gene information for PRAP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,332 Da
NCBI Official Full Name
proline-rich acidic protein 1 isoform 1
NCBI Official Synonym Full Names
proline-rich acidic protein 1
NCBI Official Symbol
PRAP1  [Similar Products]
NCBI Official Synonym Symbols
UPA; PRO1195
  [Similar Products]
NCBI Protein Information
proline-rich acidic protein 1
UniProt Protein Name
Proline-rich acidic protein 1
UniProt Synonym Protein Names
Epididymis tissue protein Li 178; Uterine-specific proline-rich acidic protein
UniProt Gene Name
PRAP1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
PRAP1_HUMAN
UniProt Comments for PRAP1
PRAP1: May play an important role in maintaining normal growth homeostasis in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: extracellular region

Molecular Function: protein binding
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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