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PRDX1 elisa kit :: Human PRDX1 ELISA Kit

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Catalog # MBS2502078
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Typical Testing Data/Standard Curve (for reference only)
Product Name

PRDX1, ELISA Kit

Popular Item
Full Product Name

Human PRDX1 (Peroxiredoxin 1) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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MBS2502078 COA
OMIM
176763
3D Structure
ModBase 3D Structure for Q06830
Species Reactivity
Specificity
This kit recognizes natural and recombinantHumanPRDX1. No significant cross-reactivity or interference between HumanPRDX1 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
15.625-1000pg/mL
Sensitivity
Min: 9.375pg/mL; Max: 1000pg/mL
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PRDX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PRDX1purchase
MBS2502078 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PRDX1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRDX1. The ELISA analytical biochemical technique of the MBS2502078 kit is based on PRDX1 antibody-PRDX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRDX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRDX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PRDX1 elisa kit
Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanPRDX1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PRDX1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PRDX1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PRDX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PRDX1.You can calculate the concentration of PRDX1in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PRDX1 elisa kit
PRDX1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2502078. Click to request current manual
NCBI/Uniprot data below describe general gene information for PRDX1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,110 Da
NCBI Official Full Name
peroxiredoxin-1
NCBI Official Synonym Full Names
peroxiredoxin 1
NCBI Official Symbol
PRDX1  [Similar Products]
NCBI Official Synonym Symbols
PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2; NKEF-A
  [Similar Products]
NCBI Protein Information
peroxiredoxin-1; natural killer cell-enhancing factor A; natural killer-enhancing factor A; proliferation-associated gene A; proliferation-associated gene protein; thioredoxin peroxidase 2; thioredoxin-dependent peroxide reductase 2
UniProt Protein Name
Peroxiredoxin-1
UniProt Synonym Protein Names
Natural killer cell-enhancing factor A; NKEF-A; Proliferation-associated gene protein; PAG; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2
Protein Family
UniProt Gene Name
PRDX1  [Similar Products]
UniProt Synonym Gene Names
PAGA; PAGB; TDPX2; NKEF-A; PAG  [Similar Products]
UniProt Entry Name
PRDX1_HUMAN
NCBI Summary for PRDX1
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011]
UniProt Comments for PRDX1
PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.

Protein type: Nuclear receptor co-regulator; EC 1.11.1.15; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: extracellular space; peroxisomal matrix; mitochondrial matrix; cytoplasm; melanosome; nucleolus; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; peroxidase activity; thioredoxin peroxidase activity; heme binding

Biological Process: cell proliferation; regulation of stress-activated MAPK cascade; retinal homeostasis; erythrocyte homeostasis; response to reactive oxygen species; removal of superoxide radicals; hydrogen peroxide catabolic process; natural killer cell mediated cytotoxicity; skeletal development; regulation of NF-kappaB import into nucleus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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