• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PRDX1 elisa kit :: Mouse peroxiredoxin 1 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS907485
Unit / Price
  24-Strip-Wells (LIMIT 1)  /  $240 +1 FREE 8GB USB
  48-Strip-Wells  /  $495 +1 FREE 8GB USB
  96-Strip-Wells  /  $710 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,705 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,145 +6 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

peroxiredoxin 1 (PRDX1), ELISA Kit

Popular Item
Also Known As

Mouse Peroxiredoxin-1, PRDX1 ELISA Kit

Product Synonym Names
Mouse Peroxiredoxin-1 (PRDX1) ELISA kit; MSP23; NKEFA; PAG; PAGA; PAGB; PRX1; PRXI; TDPX2; natural killer-enhancing factor A; proliferation-associated gene A; thioredoxin-dependent peroxide reductase 2; peroxiredoxin 1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
MBS907485 Testing Data
Sequence Length
199
3D Structure
ModBase 3D Structure for P35700
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse PRDX1. No significant cross-reactivity or interference between mouse PRDX1 and analogues was observed.
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0.312 ng/ml -20 ng/ml.
Sensitivity
The minimum detectable dose of mouse PRDX1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PRDX1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPRDX1purchase
MBS907485 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the peroxiredoxin 1 (PRDX1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRDX1. The ELISA analytical biochemical technique of the MBS907485 kit is based on PRDX1 antibody-PRDX1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRDX1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRDX1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PRDX1 elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRDX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Typical Testing Data/Standard Curve (for reference only) of PRDX1 elisa kit
PRDX1 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS907485. Click to request current manual
NCBI/Uniprot data below describe general gene information for PRDX1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,176 Da
NCBI Official Full Name
peroxiredoxin-1
NCBI Official Synonym Full Names
peroxiredoxin 1
NCBI Official Symbol
Prdx1  [Similar Products]
NCBI Official Synonym Symbols
PAG; OSF3; Paga; PrxI; TDX2; TPxA; prx1; MSP23; NkefA; OSF-3; PrdxI; Tdpx2
  [Similar Products]
NCBI Protein Information
peroxiredoxin-1; thioredoxin peroxidase 2; osteoblast specific factor 3; osteoblast-specific factor 3; macrophage 23 Kd stress protein; macrophage 23kDa stress protein; macrophase stress protein 22kDa; macrophase stress protein 23 kd; proliferation-associated gene A; macrophage 23 kDa stress protein; Trx dependent peroxide reductase 2; thioredoxin dependent peroxide reductase 2; thioredoxin-dependent peroxide reductase 2
UniProt Protein Name
Peroxiredoxin-1
UniProt Synonym Protein Names
Macrophage 23 kDa stress protein; Osteoblast-specific factor 3; OSF-3; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2
Protein Family
UniProt Gene Name
Prdx1  [Similar Products]
UniProt Synonym Gene Names
Msp23; Paga; Tdpx2; OSF-3  [Similar Products]
UniProt Entry Name
PRDX1_MOUSE
UniProt Comments for PRDX1
PRDX1: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression.

Protein type: Oxidoreductase; Nuclear receptor co-regulator; EC 1.11.1.15

Cellular Component: peroxisomal matrix; extracellular space; mitochondrion; mitochondrial matrix; cytoplasm; nucleolus; cytosol; nucleus; myelin sheath

Molecular Function: antioxidant activity; identical protein binding; protein binding; peroxidase activity; protein homodimerization activity; peroxiredoxin activity; thioredoxin peroxidase activity; heme binding; oxidoreductase activity

Biological Process: regulation of stress-activated MAPK cascade; erythrocyte homeostasis; cell proliferation; response to reactive oxygen species; removal of superoxide radicals; natural killer cell mediated cytotoxicity; hydrogen peroxide catabolic process; response to oxidative stress; regulation of NF-kappaB import into nucleus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000