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PRDX3 elisa kit :: Human Peroxiredoxin 3 (PRDX3) ELISA Kit

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Catalog # MBS2601074
Unit / Price
  48-Strip-Wells  /  $260 +1 FREE 8GB USB
  96-Strip-Wells  /  $405 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $1,615 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $2,880 +3 FREE 8GB USB
Typical Standard Curve/Testing Data
Product Name

Peroxiredoxin 3 (PRDX3), ELISA Kit

Popular Item
Also Known As

Human Peroxiredoxin 3 (PRDX3) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
256
OMIM
604769
Species Reactivity
Specificity
No cross-reaction with other factors.
Samples
Serum, plasma or cell culture supernatant
Assay Type
Quantitative Sandwich
Detection Range
200 ng/ml-0.312 ng/ml
Sensitivity
Up to 0.06 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PRDX3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPRDX3purchase
MBS2601074 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Peroxiredoxin 3 (PRDX3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRDX3. The ELISA analytical biochemical technique of the MBS2601074 kit is based on PRDX3 antibody-PRDX3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRDX3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRDX3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PRDX3 elisa kit
Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human PRDX3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

Typical Standard Curve/Testing Data of PRDX3 elisa kit
PRDX3 elisa kit Typical Standard Curve/Testing Data image
Sample Manual Insert of MBS2601074. Click to request current manual
NCBI/Uniprot data below describe general gene information for PRDX3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,839 Da
NCBI Official Full Name
Peroxiredoxin 3
NCBI Official Synonym Full Names
peroxiredoxin 3
NCBI Official Symbol
PRDX3  [Similar Products]
NCBI Official Synonym Symbols
AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III
  [Similar Products]
NCBI Protein Information
thioredoxin-dependent peroxide reductase, mitochondrial; peroxiredoxin-3; peroxiredoxin III; protein MER5 homolog; antioxidant protein 1
UniProt Protein Name
Thioredoxin-dependent peroxide reductase, mitochondrial
UniProt Synonym Protein Names
Antioxidant protein 1; AOP-1; HBC189; Peroxiredoxin III; Prx-III; Peroxiredoxin-3; Protein MER5 homolog
UniProt Gene Name
PRDX3  [Similar Products]
UniProt Synonym Gene Names
AOP1; AOP-1; Prx-III  [Similar Products]
UniProt Entry Name
PRDX3_HUMAN
NCBI Summary for PRDX3
This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for PRDX3
PRDX3: Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical- generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Belongs to the AhpC/TSA family.

Protein type: Oxidoreductase; EC 1.11.1.15

Chromosomal Location of Human Ortholog: 10q25-q26

Cellular Component: mitochondrion; mitochondrial matrix; early endosome; cytoplasm; IkappaB kinase complex; cytosol

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; thioredoxin peroxidase activity; caspase inhibitor activity; alkyl hydroperoxide reductase activity; protein kinase binding; kinase binding

Biological Process: mitochondrion organization and biogenesis; negative regulation of kinase activity; response to lipopolysaccharide; negative regulation of caspase activity; activation of NF-kappaB transcription factor; response to reactive oxygen species; peptidyl-cysteine oxidation; regulation of mitochondrial membrane potential; response to hydrogen peroxide; hydrogen peroxide catabolic process; positive regulation of cell proliferation; myeloid cell differentiation; response to oxidative stress; negative regulation of apoptosis; maternal placenta development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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