• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

PRG4 elisa kit :: Guinea Pig PRG4 ELISA Kit

Scan QR to view Datasheet
Catalog # MBS2509591
Unit / Price
  48-Strip-Wells  /  $450 +1 FREE 8GB USB
  96-Strip-Wells  /  $535 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $2,255 +2 FREE 8GB USB
  10x96-Strip-Wells  /  $3,975 +4 FREE 8GB USB
Typical Testing Data/Standard Curve (for reference only)
Product Name

PRG4, ELISA Kit

Popular Item
Also Known As

Guinea pig PRG4 (Proteoglycan 4) ELISA Kit

Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
1404
OMIM
208250
3D Structure
ModBase 3D Structure for Q92954
Species Reactivity
Specificity
This kit recognizes natural and recombinant Guinea pig PRG4. No significant cross-reactivity or interference between Guinea pig PRG4 and analogues was observed.
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
1.563-100ng/mL
Sensitivity
Min: 0.938ng/mL; Max: 100ng/mL
Intended Uses
This ELISA kit applies to the in vitro quantitative determination of Guinea pig PRG4 concentrations in serum, plasma and other biological fluids.
Preparation and Storage
Store at 4 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PRG4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPRG4purchase
MBS2509591 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PRG4, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRG4. The ELISA analytical biochemical technique of the MBS2509591 kit is based on PRG4 antibody-PRG4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRG4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRG4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PRG4 elisa kit
Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PRG4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PRG4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PRG4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PRG4. You can calculate the concentration of PRG4 in the samples by comparing the OD of the samples to the standard curve.

Typical Testing Data/Standard Curve (for reference only) of PRG4 elisa kit
PRG4 elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2509591. Click to request current manual
NCBI/Uniprot data below describe general gene information for PRG4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
146,468 Da
NCBI Official Full Name
proteoglycan 4 isoform A
NCBI Official Synonym Full Names
proteoglycan 4
NCBI Official Symbol
NCBI Official Synonym Symbols
MSF; SZP; CACP; HAPO; JCAP
  [Similar Products]
NCBI Protein Information
proteoglycan 4; articular superficial zone protein; hemangiopoietin; lubricin; megakaryocyte stimulating factor; superficial zone proteoglycan
UniProt Protein Name
Proteoglycan 4
UniProt Synonym Protein Names
Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycanProteoglycan 4 C-terminal part
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MSF; SZP  [Similar Products]
UniProt Entry Name
PRG4_HUMAN
NCBI Summary for PRG4
The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
UniProt Comments for PRG4
PRG4: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Defects in PRG4 are the cause of camptodactyly- arthropathy-coxa vara-pericarditis syndrome (CACP); also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q25-q31

Cellular Component: extracellular space

Molecular Function: scavenger receptor activity; polysaccharide binding

Biological Process: cell proliferation; receptor-mediated endocytosis; negative regulation of interleukin-6 biosynthetic process; immune response; regulation of cell proliferation

Disease: Camptodactyly-arthropathy-coxa Vara-pericarditis Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000