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PRICKLE1 elisa kit :: Human Prickle-like protein 1 ELISA Kit

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Catalog # MBS281033
Unit / Price
  48-Strip-Wells  /  $405 +1 FREE 8GB USB
  96-Strip-Wells  /  $675 +1 FREE 8GB USB
  5x96-Strip-Wells  /  $3,080 +3 FREE 8GB USB
  10x96-Strip-Wells  /  $5,805 +7 FREE 8GB USB
PRICKLE1 elisa kit
Product Name

Prickle-like protein 1 (PRICKLE1), ELISA Kit

Popular Item
Also Known As

Human Prickle-like protein 1 (PRICKLE1) ELISA Kit

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Sequence Length
AF399844 mRNA
3D Structure
ModBase 3D Structure for Q96MT3
Species Reactivity
This assay has high sensitivity and excellent specificity for detection of Human PRICKLE1. No significant cross-reactivity or interference between Human PRICKLE1 and analogues was observed.
Serum, Plasma, Other biological fluids
Assay Type
Sample Volume
1-200 uL
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PRICKLE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPRICKLE1purchase
MBS281033 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prickle-like protein 1 (PRICKLE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRICKLE1. The ELISA analytical biochemical technique of the MBS281033 kit is based on PRICKLE1 antibody-PRICKLE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRICKLE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRICKLE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PRICKLE1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PRICKLE1 in samples. An antibody specific for PRICKLE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRICKLE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRICKLE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRICKLE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Manual Insert of MBS281033. Click to request current manual
NCBI/Uniprot data below describe general gene information for PRICKLE1. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
94,300 Da
NCBI Official Full Name
prickle-like protein 1
NCBI Official Synonym Full Names
prickle planar cell polarity protein 1
NCBI Official Symbol
PRICKLE1  [Similar Products]
NCBI Official Synonym Symbols
  [Similar Products]
NCBI Protein Information
prickle-like protein 1
UniProt Protein Name
Prickle-like protein 1
UniProt Synonym Protein Names
REST/NRSF-interacting LIM domain protein 1
Protein Family
UniProt Gene Name
PRICKLE1  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for PRICKLE1
This gene encodes a nuclear receptor that may be a negative regulator of the Wnt/beta-catenin signaling pathway. The encoded protein localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in this gene have been linked to progressive myoclonus epilepsy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 3. [provided by RefSeq, Sep 2009]
UniProt Comments for PRICKLE1
PRICKLE1: Involved in the planar cell polarity pathway that controls convergent extension during gastrulation and neural tube closure. Convergent extension is a complex morphogenetic process during which cells elongate, move mediolaterally, and intercalate between neighboring cells, leading to convergence toward the mediolateral axis and extension along the anteroposterior axis. Necessary for nuclear localization of REST. May serve as nuclear receptor. Defects in PRICKLE1 are the cause of progressive myoclonic epilepsy type 1B (EPM1B). EPM1B is an autosomal recessive disorder characterized by myoclonus that progresses in severity over time, tonic-clonic seizures and ataxia. Defects in PRICKLE1 may be a cause of susceptibility to neural tube defects (NTD). Congenital malformations of the central nervous system and adjacent structures related to defective neural tube closure during the first trimester of pregnancy. Failure of neural tube closure can occur at any level of the embryonic axis. Common NTD forms include anencephaly, myelomeningocele and spina bifida, which result from the failure of fusion in the cranial and spinal region of the neural tube. NTDs have a multifactorial etiology encompassing both genetic and environmental components. Belongs to the prickle / espinas / testin family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: cytosol; nuclear membrane; nucleus

Molecular Function: protein binding; zinc ion binding

Biological Process: negative regulation of canonical Wnt signaling pathway; negative regulation of cardiac muscle cell myoblast differentiation; negative regulation of transcription, DNA-dependent; neural tube closure; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein ubiquitination; protein import into nucleus; Wnt receptor signaling pathway, planar cell polarity pathway

Disease: Epilepsy, Progressive Myoclonic 1b
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with PRICKLE1 elisa kit
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