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PRKDC blocking peptide :: DNA-PKcs Blocking Peptide

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Catalog # MBS8243983
Unit / Price
  1 mg  /  $195 +1 FREE 8GB USB
  5 mg  /  $435 +1 FREE 8GB USB
PRKDC blocking peptide
Product Name

DNA-PKcs (PRKDC), Blocking Peptide

Also Known As

DNA-PKcs (pT2647) Blocking Peptide

Product Synonym Names
HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Length
4128
OMIM
600899
3D Structure
ModBase 3D Structure for P78527
Host
Synthetic
Species Reactivity
Human, Monkey
Purity/Purification
>85%
Form/Format
Lyophilized Powder
Quality Control
The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Other Notes
Small volumes of PRKDC blocking peptide vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PRKDC blocking peptide
The peptide is used to block Anti-DNA-PKcs reactivity.
Applications Tested/Suitable for PRKDC blocking peptide
Blocking (BL)
Application Notes for PRKDC blocking peptide
Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
NCBI/Uniprot data below describe general gene information for PRKDC. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
465,501 Da
NCBI Official Full Name
DNA-dependent protein kinase catalytic subunit
NCBI Official Synonym Full Names
protein kinase, DNA-activated, catalytic polypeptide
NCBI Official Symbol
PRKDC  [Similar Products]
NCBI Official Synonym Symbols
HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
  [Similar Products]
NCBI Protein Information
DNA-dependent protein kinase catalytic subunit
UniProt Protein Name
DNA-dependent protein kinase catalytic subunit
UniProt Synonym Protein Names
DNPK1; p460
UniProt Gene Name
PRKDC  [Similar Products]
UniProt Synonym Gene Names
HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs  [Similar Products]
UniProt Entry Name
PRKDC_HUMAN
NCBI Summary for PRKDC
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
UniProt Comments for PRKDC
DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, atypical; Nucleolus; Kinase, protein; DNA repair, damage; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; DNAPK subfamily

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: cytosol; DNA-dependent protein kinase complex; membrane; nuclear chromosome, telomeric region; nucleolus; nucleoplasm; transcription factor complex

Molecular Function: ATP binding; DNA-dependent protein kinase activity; double-stranded DNA binding; enzyme binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transcription factor binding

Biological Process: B cell lineage commitment; brain development; cell proliferation; cellular response to insulin stimulus; DNA damage response, signal transduction resulting in induction of apoptosis; DNA repair; double-strand break repair; double-strand break repair via nonhomologous end joining; germ cell programmed cell death; heart development; immunoglobulin V(D)J recombination; innate immune response; negative regulation of apoptosis; negative regulation of immunoglobulin production; negative regulation of protein amino acid phosphorylation; peptidyl-serine phosphorylation; positive regulation of apoptosis; positive regulation of developmental growth; positive regulation of fibroblast proliferation; positive regulation of immune system process; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; pro-B cell differentiation; protein destabilization; protein modification process; regulation of circadian rhythm; regulation of smooth muscle cell proliferation; response to activity; response to gamma radiation; rhythmic process; somitogenesis; spleen development; T cell differentiation in the thymus; T cell lineage commitment; T cell receptor V(D)J recombination; telomere capping; thymus development

Disease: Immunodeficiency 26 With Or Without Neurologic Abnormalities
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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