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PRL elisa kit :: Bovine Prolactin (PRL) ELISA Kit

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Catalog # MBS2700354 (SPECIAL PROMOTIONAL PRICING for a limited time)
Unit / Price
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  24-Strip-Wells  /  $230 +1 FREE 8GB USB
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Typical Testing Data/Standard Curve (for reference only)
Product Name

Prolactin (PRL), ELISA Kit

Popular Item
Full Product Name

Cattle Prolactin (PRL) ELISA Kit

Product Synonym Names
LTH; Luteotropic Hormone
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
OMIM
V00566 mRNA
3D Structure
ModBase 3D Structure for P01236
Species Reactivity
Specificity
This assay has high sensitivity and excellent specificity for detection of Prolactin (PRL).
No significant cross-reactivity or interference between Prolactin (PRL) and analogues was observed.
Assay Type
Competitive Inhibition
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
2.47-200ng/mL
Sensitivity
< 0.94ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Prolactin (PRL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Prolactin (PRL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Note
Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of PRL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms forPRLpurchase
MBS2700354 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prolactin (PRL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PRL. The ELISA analytical biochemical technique of the MBS2700354 kit is based on PRL antibody-PRL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PRL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PRL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
PRL elisa kit
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Prolactin (PRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Prolactin (PRL) and unlabeled Prolactin (PRL) (Standards or samples) with the pre-coated antibody specific to Prolactin (PRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Prolactin (PRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Prolactin (PRL) in the sample.

Typical Testing Data/Standard Curve (for reference only) of PRL elisa kit
PRL elisa kit Typical Testing Data/Standard Curve (for reference only) image
Sample Manual Insert of MBS2700354. Click to request current manual
NCBI/Uniprot data below describe general gene information for PRL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,876 Da
NCBI Official Full Name
prolactin
NCBI Official Synonym Full Names
prolactin
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
prolactin
UniProt Protein Name
Prolactin
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for PRL
This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]
UniProt Comments for PRL
prolactin: Prolactin acts primarily on the mammary gland by promoting lactation. Belongs to the somatotropin/prolactin family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extracellular region

Molecular Function: hormone activity; prolactin receptor binding; protein binding

Biological Process: cell proliferation; cell surface receptor signaling pathway; cellular protein metabolic process; female pregnancy; lactation; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; positive regulation of JAK-STAT cascade; regulation of multicellular organism growth; regulation of receptor activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Organs/Tissues associated with PRL elisa kit
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