Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Active Protein rightarrow SPARC  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Interleukin 8 (IL8) Antibody
 • GAL3 ELISA Kit
 • SAMHD1 Antibody
 • Anti-Double Stranded DNA Antibody (Anti-dsDNA) ELISA Kit
 • S100A7A Antibody
 • angiopoietin-like 6 (ANGPTL6) ELISA Kit
 • Interleukin 11 (IL-11) ELISA Kit
 • Aldh3A1 Blocking Peptide
 • tyrosine 3-monooxygenase-tryptophan 5-monooxygenase activati... Antibody
 • a-Internexin-NF66 (Ina) Antibody
 • ATF2 Antibody
 • Crossveinless 2 (BMPER) Antibody
 • myelin basic protein (MBP) ELISA Kit
 • Fibroblast growth factor 19 protein (FGF19) Active Protein
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

SPARC active protein :: SPARC protein (SPARC) Active Protein

Scan QR to view Datasheet Catalog #    MBS969149
SDS-PAGE
Unit / Price
0.01 mg  /  $115 +1 FREE 8GB USB
0.05 mg  /  $205 +1 FREE 8GB USB
0.1 mg  /  $230 +1 FREE 8GB USB
0.25 mg  /  $380 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SPARC protein (SPARC), Active Protein

★Popular Item★
 Also Known As   

Recombinant Human SPARC protein (SPARC) (Active)

 Product Synonym Names    Basement-membrane protein 40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
 Product Gene Name   

SPARC active protein

[Similar Products]
 Product Synonym Gene Name    ON [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Sequence Positions    18-303aa, Full Length of Mature Protein
 Sequence    MSYYHHHHHH DYDIPTTENL YFQGAMGSAP QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI
 OMIM    Y00755 mRNA
 3D Structure    ModBase 3D Structure for P09486
Section Bar
 Host    E Coli
Section Bar
 Purity/Purification    >=95% as determined by SDS-PAGE and HPLC. (lot specific)
 Form/Format    Lyophilized
Section Bar
 Species    Homo sapiens (Human)
 Biological Activity    Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of > 333 IU/mg.
 Storage Buffer    0.2 um filtered PBS, pH 7.4,lyophilized
 Endotoxin    Less than 1.0 EU/ug as determined by LAL method.
 Protein Note    Protein will be provided with aseptic processing and endotoxin removal.
Section Bar
 Preparation and Storage    Generally, the shelf life of liquid form is 6 months at -20/-80 degree C. The shelf life of lyophilized form is 12 months at -20/-80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Active Protein Note    Refer to the datasheet for activity details. For other activity applications, researchers should evaluate this protein for suitability in their model system.
 Other Notes    Small volumes of SPARC active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for SPARC active protein

   Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
 Product Categories/Family for SPARC active protein    Biological Active Protein; Cancer
Section Bar
 SDS-PAGE of SPARC active protein    SPARC active protein SDS-PAGE image
(Note: Representative image, actual molecular weight may vary depending on Tag type and expression host)
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for SPARC. It may not necessarily be applicable to this product.
 NCBI GI #    827342585
 NCBI GeneID    6678
 NCBI Accession #    NP_001296372.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001309443.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P09486 [Other Products]
 UniProt Secondary Accession #    Q6IBK4; D3DQH9 [Other Products]
 UniProt Related Accession #    P09486 [Other Products]
 Molecular Weight    36.1 kDa
Table BarTOPTable Bar
 NCBI Official Full Name    SPARC isoform 2
 NCBI Official Synonym Full Names    secreted protein acidic and cysteine rich
 NCBI Official Symbol    SPARC [Similar Products]
 NCBI Official Synonym Symbols   
ON; OI17; BM-40
[Similar Products]
 NCBI Protein Information    SPARC
 UniProt Protein Name    SPARC
 UniProt Synonym Protein Names   
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
 Protein Family    SPARC
 UniProt Gene Name    SPARC [Similar Products]
 UniProt Synonym Gene Names    ON; BM-40; ON [Similar Products]
Section Bar
 NCBI Summary for SPARC    This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
Section Bar
 UniProt Comments for SPARC    SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; mitochondrion; nuclear matrix; plasma membrane; platelet alpha granule membrane

Molecular Function: calcium ion binding; collagen binding; extracellular matrix binding; protein binding

Biological Process: extracellular matrix organization and biogenesis; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; platelet degranulation; receptor-mediated endocytosis; regulation of cell morphogenesis

Disease: Osteogenesis Imperfecta, Type Xvii
Table BarTOPTable Bar
 Research Articles on SPARC    1. SPARC can serve a dual function role as both predictor for prognosis and potentially biomarker for lymph node metastasis in resected pancreatic cancer patients.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with SPARC active proteinPathways associated with SPARC active protein
 Reference Product  PubMed Publications
 TGFB1 active protein  >74 publications with SPARC and TGFB1
 COL1A1 active protein  >36 publications with SPARC and COL1A1
 COL3A1 active protein  >24 publications with SPARC and COL3A1
 FN1 active protein  >17 publications with SPARC and FN1
 VEGFA active protein  >9 publications with SPARC and VEGFA
 TIMP1 active protein  >8 publications with SPARC and TIMP1
 VWF active protein  >7 publications with SPARC and VWF
 PLG active protein  >2 publications with SPARC and PLG
 ALB active protein  >1 publications with SPARC and ALB
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Scavenging By Class H Receptors Pathway antibodies  Scavenging By Class H Receptors Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
Diseases associated with SPARC active proteinOrgans/Tissues associated with SPARC active protein
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >114 publications with SPARC and Inflammation
 Disease Models, Animal Antibodies  >112 publications with SPARC and Disease Models, Animal
 Fibrosis Antibodies  >103 publications with SPARC and Fibrosis
 Breast Neoplasms Antibodies  >84 publications with SPARC and Breast Neoplasms
 Brain Diseases Antibodies  >77 publications with SPARC and Brain Diseases
 Necrosis Antibodies  >68 publications with SPARC and Necrosis
 Neoplasms, Experimental Antibodies  >55 publications with SPARC and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >52 publications with SPARC and Lung Neoplasms
 Cell Transformation, Neoplastic Antibodies  >50 publications with SPARC and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >46 publications with SPARC and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1129 publications with SPARC and Bone
 Connective Tissue Antibodies  >461 publications with SPARC and Connective Tissue
 Blood Antibodies  >347 publications with SPARC and Blood
 Vascular Antibodies  >315 publications with SPARC and Vascular
 Bone Marrow Antibodies  >214 publications with SPARC and Bone Marrow
 Muscle Antibodies  >200 publications with SPARC and Muscle
 Embryonic Tissue Antibodies  >180 publications with SPARC and Embryonic Tissue
 Mouth Antibodies  >168 publications with SPARC and Mouth
 Brain Antibodies  >157 publications with SPARC and Brain
 Heart Antibodies  >126 publications with SPARC and Heart
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions