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VEGF-165 active protein

Scan QR to view Datasheet Catalog #    MBS506313
Gel Data
Unit / Price
0.002 mg  /  $140 +1 FREE 8GB USB
0.01 mg  /  $225 +1 FREE 8GB USB
0.1 mg  /  $510 +1 FREE 8GB USB
0.5 mg  /  $2,290 +2 FREE 8GB USB
1 mg  /  $3,750 +4 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VEGF-165, Active Protein

★Popular Item★
 Also Known As   

Recombinant Rat VEGF-165

 Product Synonym Names    Rat VEGF-165; Vascular Endothelial Growth Factor-165; VEGF-A; VPF; glioma-derived endothelial cell mitogen
 Product Gene Name   

VEGF-165 active protein

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Sequence    MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
 3D Structure    ModBase 3D Structure for P16612
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 Host    E Coli
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 Purity/Purification    Purity is typically 97% as determined by HPLC, reducing and non-reducing SDS-PAGE, UV spectroscopy at 280 nm
 Form/Format    Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing
10 mM sodium phosphate, pH 7.5
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 Species    Rat
 Bioactivity Assay    bioactive protein but no acceptaince criteria limits established
 Reconstitution    Sterile water at 0.1 mg/mL
 Acceptance Criteria LAL (EUs/ug)    <=1.00
 Monomer/Dimer    Monomer
 Endotoxin Level (kinetic LAL analysis)    Typically less than 0.05 EUs/mug protein (50% confidence range)
 Biological Activity    The activity is determined by the dose-dependent induced proliferation of Human umbilical vein endothelial cells (HUVECs) and is typically 4.0-8.0 ng/mL. This corresponds to an expected specific activity of 2.5 x 105 units/mg.
 Media Components    Luria Broth
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 Preparation and Storage    12 months from date of receipt when stored at -20 degree C to-80 degree C as supplied.
1 month when stored at 4 degree C after reconstituting as directed.
3 months when stored at -20 degree C to -80 degree C after reconstituting as directed.
 Active Protein Note    Refer to the datasheet for activity details. For other activity applications, researchers should evaluate this protein for suitability in their model system.
 Other Notes    Small volumes of VEGF-165 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for VEGF-165 active protein

   Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant. The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.
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 Gel Data of VEGF-165 active protein    VEGF-165 active protein Gel Data image
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NCBI/Uniprot data below describe general gene information for VEGF-165. It may not necessarily be applicable to this product.
 NCBI GI #    160358797
 NCBI GeneID    83785
 NCBI Accession #    NP_001103804.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001110334.2 [Other Products]
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 UniProt Primary Accession #    P16612 [Other Products]
 UniProt Secondary Accession #    Q541S6; Q91ZE1; Q9JKX7; Q9QXG6; Q9QXG7 [Other Products]
 UniProt Related Accession #    P16612 [Other Products]
 Molecular Weight    Non-glycosylated, disulfide-linked homodimer, containing two 165 amino acids chains, with a total molecular weight of 38.8 kDa.
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 NCBI Official Full Name    vascular endothelial growth factor A isoform 3
 NCBI Official Synonym Full Names    vascular endothelial growth factor A
 NCBI Official Symbol    Vegfa [Similar Products]
 NCBI Official Synonym Symbols   
VPF; Vegf; VEGF-A; VEGF111; VEGF164
[Similar Products]
 NCBI Protein Information    vascular endothelial growth factor A
 UniProt Protein Name    Vascular endothelial growth factor A
 UniProt Synonym Protein Names   
Vascular permeability factor; VPF
 UniProt Gene Name    Vegfa [Similar Products]
 UniProt Synonym Gene Names    Vegf; VEGF-A; VPF [Similar Products]
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 NCBI Summary for VEGF-165    This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]
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 UniProt Comments for VEGF-165    VEGF: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the PDGF/VEGF growth factor family. 13 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q12

Cellular Component: basement membrane; cell surface; cytoplasm; extracellular region; extracellular space; nucleus; secretory granule; vesicle

Molecular Function: chemoattractant activity; cytokine activity; extracellular matrix binding; fibronectin binding; growth factor activity; growth factor binding; heparin binding; identical protein binding; platelet-derived growth factor receptor binding; protein heterodimerization activity; protein homodimerization activity; receptor agonist activity; receptor binding; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 2 binding; vascular endothelial growth factor receptor binding

Biological Process: activation of CREB transcription factor; activation of protein kinase activity; aging; alveolus development; angiogenesis; artery morphogenesis; basophil chemotaxis; blood vessel development; blood vessel endothelial cell proliferation during sprouting angiogenesis; blood vessel morphogenesis; blood vessel remodeling; branching morphogenesis of a tube; camera-type eye morphogenesis; cardiac muscle fiber development; cell maturation; cell migration; cell migration during sprouting angiogenesis; cell proliferation; endothelial cell migration; epithelial cell differentiation; eye photoreceptor cell development; female pregnancy; growth; heart morphogenesis; homeostasis of number of cells within a tissue; hyaluronan metabolic process; in utero embryonic development; induction of positive chemotaxis; kidney development; lactation; lumen formation; lung development; lymphangiogenesis; macrophage differentiation; mesoderm development; monocyte differentiation; mRNA stabilization; negative regulation of apoptosis; negative regulation of bone resorption; negative regulation of caspase activity; negative regulation of cell-cell adhesion; negative regulation of fat cell differentiation; negative regulation of programmed cell death; negative regulation of transcription from RNA polymerase II promoter; nervous system development; ovarian follicle development; patterning of blood vessels; positive chemotaxis; positive regulation of angiogenesis; positive regulation of axon extension involved in axon guidance; positive regulation of blood vessel endothelial cell migration; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of cell motility; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of focal adhesion formation; positive regulation of MAP kinase activity; positive regulation of mesenchymal cell proliferation; positive regulation of neuroblast proliferation; positive regulation of osteoblast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of positive chemotaxis; positive regulation of protein amino acid autophosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of receptor internalization; positive regulation of signal transduction; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of vascular permeability; post-embryonic camera-type eye development; regulation of cell shape; regulation of cGMP metabolic process; regulation of endothelial cell differentiation; regulation of transcription from RNA polymerase II promoter; response to cold; response to estradiol stimulus; response to folic acid; response to hypoxia; response to progesterone stimulus; response to vitamin A; surfactant homeostasis; T-helper 1 type immune response; vascular endothelial growth factor receptor signaling pathway; vasculature development; wound healing
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 Research Articles on VEGF-165    1. Treatment with NaHS prevents hypertension in pregnancy and concomitantly reduces circulating plasma sFlt-1 and VEGF levels; this correlates with improved litter size with more viable fetuses and increase in NO levels.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with VEGF-165 active proteinPathways associated with VEGF-165 active protein
 Reference Product  PubMed Publications
 Hif1a active protein  >697 publications with VEGF-165 and Hif1a
 Kdr active protein  >411 publications with VEGF-165 and Kdr
 Flt1 active protein  >215 publications with VEGF-165 and Flt1
 Tgfb1 active protein  >189 publications with VEGF-165 and Tgfb1
 Mmp9 active protein  >163 publications with VEGF-165 and Mmp9
 Pgf active protein  >139 publications with VEGF-165 and Pgf
 Nrp1 active protein  >60 publications with VEGF-165 and Nrp1
 Flt4 active protein  >36 publications with VEGF-165 and Flt4
 Arnt active protein  >18 publications with VEGF-165 and Arnt
 Hif3a active protein  >9 publications with VEGF-165 and Hif3a
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
 EPHA-mediated Growth Cone Collapse Pathway antibodies  EPHA-mediated Growth Cone Collapse Pathway Diagram
Diseases associated with VEGF-165 active proteinOrgans/Tissues associated with VEGF-165 active protein
 Disease Name  Pubmed Publications
 Neovascularization, Pathologic Antibodies  >2173 publications with VEGF-165 and Neovascularization, Pathologic
 Retinal Diseases Antibodies  >1754 publications with VEGF-165 and Retinal Diseases
 Neoplasm Metastasis Antibodies  >552 publications with VEGF-165 and Neoplasm Metastasis
 Diabetic Retinopathy Antibodies  >543 publications with VEGF-165 and Diabetic Retinopathy
 Inflammation Antibodies  >541 publications with VEGF-165 and Inflammation
 Diabetes Complications Antibodies  >462 publications with VEGF-165 and Diabetes Complications
 Lung Diseases Antibodies  >459 publications with VEGF-165 and Lung Diseases
 Macular Edema Antibodies  >442 publications with VEGF-165 and Macular Edema
 Heart Diseases Antibodies  >311 publications with VEGF-165 and Heart Diseases
 Head and Neck Neoplasms Antibodies  >290 publications with VEGF-165 and Head and Neck Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1703 publications with VEGF-165 and Eye
 Lung Antibodies  >591 publications with VEGF-165 and Lung
 Muscle Antibodies  >493 publications with VEGF-165 and Muscle
 Embryonic Tissue Antibodies  >444 publications with VEGF-165 and Embryonic Tissue
 Heart Antibodies  >416 publications with VEGF-165 and Heart
 Liver Antibodies  >391 publications with VEGF-165 and Liver
 Brain Antibodies  >364 publications with VEGF-165 and Brain
 Placenta Antibodies  >261 publications with VEGF-165 and Placenta
 Connective Tissue Antibodies  >204 publications with VEGF-165 and Connective Tissue
 Colon Antibodies  >187 publications with VEGF-165 and Colon
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