View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Endodermal Lineage Marker Antibody Kit Catalog # MBS2550137 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Endodermal Lineage Marker, Antibody Kit Popular Item Full Product Name Endodermal Lineage Marker Antibody Sampler Kit Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Sample Manual Insert Download Sample PDF Manual View Sample PDF Manual ISO Certification Manufactured in an ISO 9001:2015 Certified Laboratory. Product Note Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details. Other Notes Small volumes of Endodermal Lineage Marker antibody kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for Endodermal Lineage Marker antibody kit Background: Two endodermal lineages develop during mammalian embryogenesis, the primitive endoderm of the blastocyst stage embryo and the definitive endoderm at gastrulation. The primitive endoderm gives rise to extra- embryonic lineages encompassing the visceral and the parietal endoderm. The definitive endoderm contributes to the respiratory and gastrointestinal tracts by forming the epithelial lining of the trachea, esophagus, lungs, stomach and intestines, and is a major component of many glands, including thyroid, thymus, pancreas and liver. Understanding molecular mechanisms that regulate early endodermal fates is seminal for the advance of stem cell research as they connect the transition from pluripotency to endoderm specification during mammalian development and contribute to the generation of clinically relevant cell types. FoxA2/HNF3beta is a transcription factor essential for development of the endoderm and midline structures in mouse embryos. EOMES acts during gastrulation to promote the specification of the definitive endoderm. Markers of hepatic differentiation in the endoderm include expression of alpha-fetoprotein (AFP) and N-cadherin. HNF4alpha is involved in the differentiation of the visceral endoderm. GATA-6 lies upstream of HNF4 in a transcriptional cascade that regulates differentiation of the visceral endoderm and is also required for the establishment of the endodermally derived bronchial epithelium. Sall4 is required for the formation of the primitive endoderm from inner cell mass. It has been reported that extra-embryonic stem cell lines cannot be formed in Sall4-deficient blastocysts. PDGF receptor alpha is expressed in primitive endoderm derivatives throughout embryogenesis. Applications Tested/Suitable for Endodermal Lineage Marker antibody kit Western Blot (WB) Western Blot (WB) of Endodermal Lineage Marker antibody kit Western Blot analysis of Mouse liver, Mouse serum with AFP Polyclonal Antibody. Western Blot (WB) of Endodermal Lineage Marker antibody kit Western Blot analysis of HT29 cells with EOMES Polyclonal Antibody. Western Blot (WB) of Endodermal Lineage Marker antibody kit Western Blot analysis of Lovo cell using FOXA2 Polyclonal Antibody at dilution of 1:500 Western Blot (WB) of Endodermal Lineage Marker antibody kit Western Blot analysis of HT29 cells using HNF-4alpha Polyclonal Antibody at dilution of 1:2000. Western Blot (WB) of Endodermal Lineage Marker antibody kit Western Blot analysis of various cells using N-cadherin Polyclonal Antibody at dilution of 1:1000. Western Blot (WB) of Endodermal Lineage Marker antibody kit Western Blot analysis of HepG2 cells with PDGFR-alpha Polyclonal Antibody. Western Blot (WB) of Endodermal Lineage Marker antibody kit Western Blot analysis of K-562 cells using SALL4 Monoclonal Antibody at dilution of 1:1000 TOP Sample Manual Insert of MBS2550137. Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP