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anti-AMPK-alpha (pT172) antibody :: Rabbit AMPK-alpha (pT172) Antibody

Scan QR to view Datasheet Catalog #    MBS462009
Testing Data
Unit / Price
0.1 mg  /  $375 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

AMPK-alpha (pT172) (AMPK-alpha (pT172)), Antibody

 Also Known As   

Rabbit anti Phospho- AMPK alpha 1 (pThr 172)

 Product Synonym Names    Rabbit anti--AMPK alpha 1 (pT172) Phosphospecific; AMPKalpha-1; AMP-activated protein kinase alpha 1;PRKAA1; MGC57364; AMPK
 Product Gene Name   

anti-AMPK-alpha (pT172) antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Host    Rabbit
 Species Reactivity    Human, Rat, Mouse, Bovine, Canine, Chicken
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 Specificity    This antibody recognizes AMPK alpha with a phosphorylated site of Thr 172. It does not cross-react with non-phosphospecific peptide.
 Purity/Purification    The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
 Form/Format    This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
 Concentration    Size: 100 ug/200 ul (lot specific)
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 Antigen Preparation    A synthetic peptide surrounding to the epitope -LRTSC- with a phosphorylation site at Thr 172 of AMPK alpha 1 protein from human origin. It is identical among human, mouse, rat, chicken, bovine, dog, and insect species.
 Positive Control    Serum starved HEK293
 Cellular Location    Cytoplasmic
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 Preparation and Storage    The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
 Other Notes    Small volumes of anti-AMPK-alpha (pT172) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-AMPK-alpha (pT172) antibody

   AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy balance at both the cellular and the whole body levels. Once activated, it affects a metabolic switch from an anabolic to a catabolic state, both by acutely phosphorylating metabolic enzymes and, in the longer term, by regulating gene expression. AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits. Binding APM to the beta domains triggers increased phosphorylation at Thr172 on the activation loop of the alpha subunit. AMP Phosphorylation at Thr172 is catalyzed by the tumor suppressor kinase LKB1 or CaMKK-beta, TGF-beta activated kinase-1 (TAK1). AMPKalpha is also phosphorylated at Thr258 and Ser485 (for alpha1; Ser491 for alpha2).
 Applications Tested/Suitable for anti-AMPK-alpha (pT172) antibody   

ELISA, Western Blot, Immunohistochemistry

 Application Notes for anti-AMPK-alpha (pT172) antibody    Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
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 Testing Data of anti-AMPK-alpha (pT172) antibody    anti-AMPK-alpha (pT172) antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for AMPK-alpha (pT172). It may not necessarily be applicable to this product.
 NCBI GI #    60459958
 Molecular Weight    63 kDa
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 NCBI Official Full Name    AMPK-alpha subunit
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Product References and Citations for anti-AMPK-alpha (pT172) antibody

   Jing Chen et al. AMPK regulation of mouse oocyte meiotic resumption in vitro. Developmental Biology, 291 (2), 227-238 (2006).
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-AMPK-alpha (pT172) antibodyPathways associated with anti-AMPK-alpha (pT172) antibody
 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with AMPK-alpha (pT172) and FLNA
 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
Organs/Tissues associated with anti-AMPK-alpha (pT172) antibody
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with AMPK-alpha (pT172) and Bone
 Connective Tissue Antibodies  >4 publications with AMPK-alpha (pT172) and Connective Tissue
 Heart Antibodies  >3 publications with AMPK-alpha (pT172) and Heart
 Muscle Antibodies  >3 publications with AMPK-alpha (pT172) and Muscle
 Brain Antibodies  >3 publications with AMPK-alpha (pT172) and Brain
 Vascular Antibodies  >2 publications with AMPK-alpha (pT172) and Vascular
 Embryonic Tissue Antibodies  >2 publications with AMPK-alpha (pT172) and Embryonic Tissue
 Ear Antibodies  >1 publications with AMPK-alpha (pT172) and Ear
 Kidney Antibodies  >1 publications with AMPK-alpha (pT172) and Kidney
 Umbilical Cord Antibodies  >1 publications with AMPK-alpha (pT172) and Umbilical Cord
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